血管紧张素原、血管紧张素Ⅱ的Ⅰ型受体基因多态性及其与原发性高血压的关系

Angiotensinogen and angiotensin Ⅱ type Ⅰ receptor gene polymorphisms in essential hypertension in Chinese

目的 :观察血管紧张素原 (AGT) M2 35 T、血管紧张素 的 型受体 (AT1R) A116 6 C基因多态性在正常人群及原发性高血压 (EH)患者中的分布。方法 :采用聚合酶链反应 (PCR)及限制性片段长度多态性分析(RFL P)方法。结果 :正常人群 (对照组 ,80例 )及 EH(95例 )患者 AGT M2 35 T的频率分别为 0 .813、0 .832 ;AT1RA116 6 C频率分别为 0 .0 5 0、0 .0 5 3。两种基因多态性在 EH患者与对照组间无显著性差异 ,但 AGT M2 35 T在 <6 0岁的 EH患者中频率显著高于≥ 6 0岁的患者和 <6 0岁对照组 ,而 AT1R A116 6 C无此差异。结论 :两种基因多态性因人种不同而不同。 AGT M2 35 T与 EH早期发病有关 ,而 AT1R A116 6 C对于

Objective:To investigate whether the angiotensinogen(AGT) M235T and the angiotensin Ⅱ type Ⅰ receptor (AT1R) A1166C polymorphisms might be implicated in essential hypertension (EH) in Chinese of Nanjing area.Method:95 patients with documented EH and 80 normal controls were studied by PCR and RFLP.Result:The frequencies of the AGT M235T allele were 0.832 、 0.813 and the frequencies of the AT1R 1166C allele were 0.053 、 0.050 in EH patients and controls respectively.The frequencies of the AGT M235T was significantly higher in the EH (<60 years) than the EH patients (≥60 years) (χ2= 4.4 ,P< 0.05 )and thecontrols (<60 years) (χ2= 12.55 ,P< 0.01 ).No statistical significance was found between the frequencies of the AT1R A1166C in the EH patients and the controls.And no correlation between the AGT M235T and AT1R 1166C alleles was detected.Conclusion:The results suggested that AGT M235T was associated with EH occured in younger patients,but there was no relationship between the AT1R A1166C polymorphisms and EH.