间断应用甲状旁腺激素对人骨髓基质干细胞的影响

Effect of intermittent treatment with parathyroid hormone on human bone marrow stromal stem cells

背景:持续应用和间断应用甲状旁腺激素以及使用量的大小对骨的效应有很大差异。目的:观察不同剂量的甲状旁腺激素间断给药对人骨髓基质干细胞增殖和分化的影响。方法:体外培养人骨髓基质干细胞,分为空白对照组和甲状旁腺激素5,20及50nmo/L组,每天作用1h,MTT法检测人骨髓基质干细胞的增殖;碱性磷酸酶活性测定检测人骨髓基质干细胞向成骨细胞的分化。结果与结论:甲状旁腺激素50nmol/L每天作用1h可显著促进人骨髓基质干细胞的增殖,同时使碱性磷酸酶升高,均显著高于其他3组。结果证实50nmo/L甲状旁腺激素间断应用可以促进人骨髓基质干细胞的增殖和向成骨细胞的分化。

BACKGROUND： Continuous and intermittent application of parathyroid hormone at different doses can cause different effects on bone. OBJECTIVE： To observe the effect of intermittent treatment with different doses of parathyroid hormone on the proliferation and differentiation of human bone marrow stromal stem cells. METHODS： Human bone marrow stromal stem cells were cultured in vitro and divided into blank control group and 5, 20 and 50 nmo/L parathyroid hormone group, and the parathyroid hormone groups were treated for 1 hour per day. MTT and alkaline phosphatase activities were used to detect the proliferation and differentiation of human bone marrow stromal stem cells to osteoblasts. RESULTS AND CONCLUSION： 50 nmol/L of parathyroid hormone treated for 1 hour per day could greatly promote the proliferation of human bone marrow stromal stem cells and could increase the alkaline phosphatase activities, and the alkaline phosphatase activities in 50 nmo/L parathyroid hormone group were higher than other three groups. Intermittent treatment with 50 nmol/L parathyroid hormone can promote the proliferation and differentiation of human bone marrow stromal stem cells.