棕榈子提取物抗肿瘤活性研究

Studies on the antitumor effect of Trachycarpus fortunei fruit extraction

目的对棕榈子提取物体外抗肿瘤活性进行初步研究。方法用乙醇回流提取棕榈子,然后依次用氯仿、乙酸乙酯和正丁醇进行萃取,并以MTT为指导,对活性部位利用大孔树脂做进一步跟踪分析;最后,通过经典显色法,对活性组分进行初步鉴定。结果 MTT结果表明,正丁醇部位对HepG2细胞株的抑制作用最强,其IC50为(24.01±2.56)μg·mL-1;然而,大孔树脂分离富集并没有明显增强抗肿瘤活性;通过经典显色法,初步确定活性组分为鞣质。结论棕榈子正丁醇层是主要的抗肿瘤活性部位,鞣质极可能为其主要活性成分,值得进一步深入研究。

OBJECTIVE To investigate the antitumor activity of Trachycarpus fortunei fruit extraction in vitro.METHODS The fruit of Trachycarpus fortunei（Hook.） H.Wendl.was extracted by refluxing with ethanol.Then the ethanol extract was extracted with chloroform,ethyl acetate and BuOH saturated with water in sequence.The active fraction was further isolated and purified by column chromatography on macroporous resin with MTT guiding.Finally,the category of the most active fraction was preliminary identified by classical coloration method.RESULTS Among the three extractions,BuOH-soulble fraction had the most noticeable inhibitory effect on the growth of HepG2 cells;However,non-antitumor enhancing effect was observed after macroporous resin treatment;Classical coloration method indicated that tannin was most likely to be the active component of the BuOH fraction.CONCLUSION The BuOH extraction from the fruit of Trachycarpus fortunei was the most antitumor active fraction,of which the tannin was the main ingredients,and worth further exploration.