脱细胞异体真皮基质与人脂肪干细胞的生物相容性

Biocompatibility of acellular dermal matrix with human adipose-derived stem cells

背景:脱细胞异体真皮基质具有优良的生物相容性和组织细胞诱导功能。目的:评价人脂肪干细胞与脱细胞异体真皮基质的生物相容性。方法:取健康成年人吸脂术后的脂肪组织分离脂肪干细胞,并行原代与传代培养,传至第3代,将细胞与脱细胞异体真皮基质联合体外培养3,7d,倒置相差显微镜和扫描电镜观察细胞在支架材料上的黏附、生长及增殖情况,并计算细胞在材料上的黏附率;XTT比色法检测细胞的生长增殖情况。结果与结论:脂肪干细胞在支架材料上分布均匀,24h内细胞开始伸展、黏附,二三天完全伸展变形,以梭形为主,呈网状排列;随着培养时间延长,支架上的细胞逐渐增多;人脂肪干细胞细胞与脱细胞异体真皮基质混合培养后平均黏附率为95.03%,并保持正常的生长增殖速度,表明支架对细胞具有良好的黏附性;脱细胞异体真皮基质材料与人脂肪干细胞复合后相容性良好。

BACKGROUND ： Acellular dermal matrix has a good biocompatibility and the inductive effect on tissues or cells. OBJECTIVE： To evaluate the biocompatibility of acellular dermal matrix with human adipose-derived stem cells （ADSCs） METHODS ： Human ADSCs were isolated from the subcutaneous adipose tissue of healthy adult after liposuction, and primary culture and subculture of human ADSCs were conducted. Human ADSCs of passage 3 were co-cultured with the acellular dermal matrix in vitro for 3 7 days. Cell growth, proliferation and adhesion on the scaffolds were observed under inverted phase contrast microscope and scanning electron microscope, and the adhesion rate was analyzed. XTT-colorimetry was used to observe the cell growth and proliferation. RESULTS AND CONCLUSION： Human ADSCs distributed evenly on the acellular dermal matrix scaffold. The cells expanded and adhered on the scaffold within 24 hours. The cells showed extensional deformation at 2 3 days, and the cell shape was spindle in main and mesh arranged. Cells on the scaffold were gradually increased as time went on. The average adherens rate was 95.03% of ADSCs co-cultured with acellular dermal matrix scaffold, and the cell growth and proliferation were normal. It is indicated that ADSCs can well attached to acellular dermal matrix scafold and normally grow and proliferate on the scaffolds. Acellular dermal matrix scaffold has a good biocompatibility with ADSCs.