胶原/透明质酸膜与明胶海绵支架材料力学及组织相容性的比较

Comparison of material mechanics and histocompatibility between collagen/hyaluronic acid membrane and gelatin sponge scaffold

背景:课题组前期实验证明胶原/透明质酸膜具有良好的力学性能和组织相容性。目的:观察复合材料胶原/透明质酸膜及明胶海绵的生物学性能。方法:应用材料复合交联的实验方法构建胶原/透明质酸膜并测定胶原/透明质酸膜、明胶海绵支架的力学性能。将支架材料种植于兔皮下,按照2,4,6,8,12周不同时间点评价材料在体内的降解情况和组织相容性。结果与结论:①成功制备了胶原/透明质酸膜。②胶原/透明质酸膜具有较好的韧性和抗张强度,明胶海绵的力学性能不够理想。③两种材料在体内的降解均符合生物材料的组织反应过程,胶原/透明质酸膜在体内12周可完全降解,明胶海绵约6周完全降解。④胶原/透明质酸膜与平滑肌细胞的黏附率高,细胞的增殖和代谢状况较好,而明胶海绵的细胞黏黏附和增殖率相对较低。说明胶原/透明质酸膜具有较好的生物学性能。

BACKGROUND： Our previous studies have confirmed that collagen/hyaluronic acid membrane has good mechanica properties and histocompatibility. OBJECTIVE： To explore the biological properties of collagen/hyaluronic acid membrane and gelatin sponge scaffold METHODS： The collagen/hyaluronic acid membrane was constructed by compound cross linking method. The mechanical property of the collagen/hyaluronic acid membrane and gelatin sponge scaffold was detected. The scaffold materials were implanted subcutaneously in rabbits; the in vivo degradation and mechanical properties were evaluated at 2, 4, 6, 8 and 12 weeks after implantation. RESULTS AND CONCLUSION： Collagen/hyaluronic acid membrane was constructed successfully. Collagen/hyaluronic acid membrane had a better toughness and tensile strength while the mechanical property of gelatin sponge was not ideal. The in vivo degradation of the two materials was compatible with the tissue reactions of biomaterials. Complete degradation of the collagen/hyaluronic acid membrane could be done within 12 weeks, while the gelatin sponge within 6 weeks. The adhesion rate between the collagen/hyaluronic acid membrane and smooth muscle cells was high; the cell proliferation and the metabolic status were good. The adhesion rate and proliferation rate of the gelatin sponge was relatively low. These findings indicate that gelatin sponge has better biological properties.