脱细胞猪角膜表面基底膜成分的检测

Detection of basement membrane components on the surface of acellular porcine cornea

背景:前期实验显示脱细胞猪角膜具有良好的组织相容性,可以支持角膜细胞和皮肤上皮细胞的生长。目的:检测脱细胞猪角膜是否保存了利于角膜上皮细胞生长的重要组织结构—基底膜。方法:利用荧光抗体对脱细胞猪角膜表面的基底膜成分(层粘蛋白和Ⅳ型胶原)进行免疫组织化学检测,荧光显微镜下观察脱细胞猪角膜表面是否保存了基底膜成分。结果与结论:免疫荧光染色显示脱细胞猪角膜前基质表面层粘蛋白和Ⅳ型胶原呈阳性表达,与新鲜猪角膜表面基底膜的荧光表达相同,表明脱细胞猪角膜保存了利于角膜上皮细胞生长的基底膜。

BACKGROUND： The previous experiments have suggested that the acellular porcine cornea stroma （APCS） has a good compatibility and can support the growth of keratocytes and skin epithelial cells. OBJECTIVE： To detect whether the important structure for the growth of corneal epithelial cells-basement membrane can be preserved on the surface of the APCS. METHODS： Fluorescent antibody was used to detect the basement membrane component （laminin and collagen IV） by immunohistochemistry. Fluorescence microscopy was observed whether the basement membrane could be preserved on the surface of the APCS. RESULTS AND CONCLUSION： Immunofluorescence staining showed that laminin and collagen IV positively expressed on the surfaces of porcine corneal stroma with the same level of fresh normal porcine corneal stroma. The results indicate the APCS can preserve the natural basement membrane component of the cornea which is beneficial to the growth of corneal epithelial cells,