颗粒活性炭对SBR反应器中好氧颗粒污泥培养的影响研究

Enhanced Aerobic Sludge Granulation in Sequencing Batch Reactor by Granular Activated Carbon Augmentation

好氧颗粒污泥培养耗时长已经成为限制其广泛应用的重要因素之一,依据"晶核假说"原理,在反应器中投加惰性核可以加快污泥好氧颗粒化进程.为了研究颗粒活性炭对于污泥好氧颗粒化进程的影响,在SBR反应器启动初期投加颗粒活性炭(SBR有效体积的1%,平均粒径为0.1—0.3mm)作为诱导核,采用扫描电镜和细菌凋亡荧光染色来表征好氧颗粒污泥.结果表明,颗粒活性炭有利于好氧颗粒污泥的形成,运行20d即获得了成熟的好氧颗粒污泥.扫描电镜结果显示,成熟的好氧颗粒污泥结构密实,微生物种类较为丰富.好氧颗粒污泥细菌凋亡荧光染色结果表明死细菌分布较为均匀,但活细菌多位于外层;胞外多聚物多重荧光染色表明蛋白质和多糖(α-吡喃葡萄糖、α-甘露糖和β-D-吡喃葡萄糖)等物质在好氧颗粒污泥内部分布较为均匀,虽然含量接近,但β-D-吡喃葡萄糖含量最多.本研究表明,在反应器中投加颗粒活性炭可以促进好氧颗粒污泥的形成.

A sequencing batch reactor（SBR） was operated to investigate the effect of granular activated carbon （ GAC ） augmentation on aerobic granulation. Aerobic granular sludge （AGS） was augmented with GAC （ mean diameter of 0.1---0.3 mm）, whose dosage was 1% of the effective volume of the SBR. The results demonstrated that GAC could enhance the sludge granulation process in SBR. The mature AGS was harvested after 20 days of cultivation. SEM analysis documented that the AGS had dense and porosity microbial structure, and contained abundant microorganisms. Live/ dead fluorescent staining showed that dead cells were distributed throughout the granules and live cells were principally found in the outer layer. The fluorescent staining of extracellular polymeric substances showed that the protein and polysaccharide （including α-mannopyranosyl, α- glucopyranosyl sugars and β-D-glucopyranose polysaccharides）were all distributed throughout the granules. The contents of protein and polysaccharide were similar to each other, however, the contents of β-D-glucopyranose polysaccharides was the largest. It can be concluded that GAC could accelerate the formation of AGS.