HPLC法比较不同工艺制备的黄连解毒汤及日本汉方黄连解毒汤中的生物碱含量

Determination of Berberine and Palmatine in Chinese Decoction of Compounded Coptis in Comparison with Japanese Kampo “Oren-gedoku-to” by HPLC

用 HPL C法测定 4种不同工艺制备的黄连解毒汤提取物粉末及 2种日本汉方颗粒剂黄连解毒汤中的小檗碱和巴马汀含量。结果表明此方法简便、快速、精密度高、回收率较好。采用此法比较了上述 6种黄连解毒汤的生物碱含量。结果是新工艺制备的提取物中生物碱含量最高 ,其中小檗碱含量分别是东洋、津村黄连解毒汤的 6倍和13.8倍 ,是传统水煎提取物的

Contents of berberine and palmatine in four powdery preparations from “Antidotal Decoction of Compounded Coptis” and two granular Japanese Kampo “Oren gedoku to” were determinated by HPLC. Results showed that powdery preparation obtained by the new production process showed a higher content of the two alkaloids than the two Japanese Kampo 6～13.8 times and about 3 times of the products obtained from the traditional water decoction process. The HPLC determination was shown to be simple, rapid, with high precision and satisfactory recovery.