黑海参多糖对β-淀粉样蛋白诱导的皮质神经元凋亡的保护作用

Protective Effects of Polysaccharide from Holothuria atra on Apoptosis of Cortical Neurons Induced by Amyloid β-Protein

体外培养大鼠皮质神经元 ,用β-淀粉样蛋白 (β- AP)诱导皮质神经元凋亡。黑海参多糖使皮质神经元存活率增加 ,使神经元的 DNA电泳图谱不出现“梯子状”,使神经元的流式细胞仪分析图上不出现凋亡峰 ,表明黑海参多糖对 β- AP诱导的皮质神经元凋亡具有保护作用。

Cortical neurons were cultured in vitro and induced to apoptosis by amyloid β protein (β AP). Polysaccharides from several TCM were screened with the apoptotic model. It was found that the polysaccharide obtained from Holothuria atra Jaegar increased the survival rate of cortical neurons in a dose dependent manner, and prevented the neuronal nuclei from shrinkage, condensation and cleavage induced by β AP. It also prevented neuronal nuclear DNA fragmentation and blocked the neuronal apoptotic peak induced by β AP. These results indicated that polysaccharide from H. atra can block cortical neuronal apoptosis induced by β AP.