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白细胞介素类及白三烯类等炎性介质对肿瘤坏死因子α产生的影响

EFFECTS OF PRO INFLAMMATORY CYTOKINES AND LEUKOTRIENS ON PRODUCTION OF TNFα FROM MURINE PERITONEAL MACROPHAGES
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摘要 目的 :研究白细胞介素类及白三烯类等炎性介质对小鼠腹腔巨噬细胞生成及分泌肿瘤坏死因子α(TNFα)的影响。方法 :用L92 9作为靶细胞的结晶紫染色法测定巨噬细胞培养上清液及胞溶部分TNFα的含量。结果 :重组鼠白细胞介素 1β(rmIL 1β)和重组人白细胞介素 8(rhIL 8)均能促进巨噬细胞产生TNFα ,其中rhIL 8有很好的剂量相关性 ,对巨噬细胞胞溶部分的TNFα无影响 ;重组人白细胞介素 6 (rhIL 6 ) ,白三烯B4 (LTB4 ) ,白三烯C4(LTC4 )及白三烯D4 (LTD4 )均不能促进巨噬细胞生成及分泌TNFα。结论 :rhIL 8和rmIL 1β能促进小鼠腹腔巨噬细胞生成TNFα。rhIL 6 ,LTB4 ,LTC4 和LTD4 AIM: To investigate production of TNFα from murine peritoneal macrophages stimulated with inflammatory mediator, such as IL 1, IL 6, IL 8, LTB 4, LTC 4 and LTD 4. METHODS: L929 cytotoxicity was used to show the level of released and cell associated TNFα in murine peritoneal macrophages measured by means of crystal violet staining assay. RESULTS: rhIL 8(40~ 4 000 U·mL -1 ) was shown to induce dose dependent increase of released TNFα from murine macrophages. rmIL 1β(0 001~1 μg·mL -1 ) was found to increase the level of released TNFα dose independently, whereas rhIL 6(10~ 10 000 U·mL -1 ), LTB 4(5 64×10 -9 ~5 64×10 -7 mol·L -1 ), LTC 4(1 73×10 -9 ~1 73×10 -7 mol·L -1 ), LTD 4(4 03×10 -9 ~4 03×10 -7 mol·L -1 ) did not induce macrophages to produce TNFα. They all can not elevate cell associated TNFα. CONCLUSION: The results suggest that rhIL 8 and rmIL 1 can induce production of TNFα from murine peritoneal macrophages, while rhIL 6, LTB 4, LTC 4 and LTD 4 can not induce production of TNFα from murine peritoneal macrophages.
机构地区 中国医学科学院
出处 《药学学报》 CAS CSCD 北大核心 2000年第4期261-264,共4页 Acta Pharmaceutica Sinica
关键词 肿瘤坏死因子Α 炎性介质 白细胞介素 白三烯 TNFα inflammatory mediator cytokines leukotriens
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参考文献3

  • 1Wen F Q,Prostaglandins Leukotrienes Essent Fatty Acids,1998年,59卷,71页
  • 2Norris J G,J Immunol,1994年,152卷,841页
  • 3Zhang M,J Immunol,1991年,7卷,245页

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