依托咪酯对大鼠肝缺血-再灌注损伤的保护作用

Protective effects of etomidate on rat liver underwent hepatic ischemia-reperfusion injury

目的探讨依托咪酯对大鼠缺血-再灌注(I-R)损伤肝脏的保护作用。方法雄性SD大鼠40只随机均分为4组:A组,I-R模型对照;B组,I-R加生理盐水2ml.kg-1.h-1处理;C组,I-R加依托咪酯0.3mg.kg-1.h-1处理;D组,假手术对照。于再灌注后30min(T1)和60min(T2)测定肝组织和血浆中内皮素1(ET-1)与一氧化氮(NO)水平以及血清ALT和AST水平,并进行肝组织形态学观察。结果 T1、T2时,A、B、C三组肝组织和血浆中ET-1均高于D组(P<0.01),但C组低于A、B两组(P<0.05),同时,肝组织和血清中NO低于D组(P<0.01),但C组高于A、B两组(P<0.05)。T1、T2时,A、B、C三组血清ALT和AST均高于D组,但C组低于A、B两组(P<0.05或P<0.01)。肝细胞形态学发生异常改变,C组的肝脏淤血较A、B两组轻,肝细胞变性坏死不明显。结论依托咪酯通过降低ET-1水平,提高NO水平,改善肝脏微循环障碍,对肝脏I-R损伤有保护作用。

Objective To investigate the protective effects of etomidate on hepatic ischemia- reperfusion（I-R） injury in rats. Methods Forty SD rats were equally randomized into four groups of A（I-R model control）, B（I-R plus normal saline treatment）, C（I-R plus etomidate 0. 3 nag · kg-1 · h-1 treatment） and D（sham operated）. Endothelin-l（ET-1） and nitric oxide（NO） in the liver and plasma and serum alanine aminotransferase（ALT） and aspartate aminotransferase（AST） were examined at 30 min and 60 rnin after reperfusion. Morphological observation of hepatic tissues was performed as well. Results At T1 and T2, the concentrations of ET-1 in hepatic tissue and plasma were higher in groups of A,B and C than those in group D（P^0. 01） ,which were lower in group C than those in groups of A and B（P^0. 05）. At T1 and T2,the concentrations of NO in hepatic tissue and plasma were lower in groups of A,B and C than those in group D（P%0. 01） ,which were higher in group C than those in groups of A and B（P%0. 05）. At T1 and T2, the concentrations of ALT and AST in serum were higher in groups of A,B and C than those in group D（P%0. 01） ,which were lower in group C than those in groups of A and B（P%0. 05 or P%0. 01）. The morphological changes of hepatocytes and blood stasis of hepatic tissues were slighter in group C than those in groups of A and B. So did the denaturalization and necrosis of hepatocytes. Conclusion Etomidate can protect rat liver from I-R injury by reducing ET-1 level, increasing NO content, and improving hepatic microcirculation.