MicroRNA-21在非小细胞肺癌中的表达及其临床意义

Expression and clinical significance of MicroRNA-21 in non-small cell lung cancer

目的探讨microRNA-21(MiR21)在非小细胞肺癌(NSCLC)患者支气管镜活检组织中的表达及其临床意义。方法用RT-PCR的方法测定31例NSCLC患者支气管镜活检组织及10例非肺癌肺组织中MiR21的表达量,分析其与临床病理资料之间的关系。结果 MiR21在NSCLC组织中的表达明显高于非肺癌组织(P<0.05),且与患者病理类型及TNM分期有关。ROC曲线下面积为0.785(95%CI:0.635-0.935);临界值取0.158时,其灵敏度和特异度分别为73.3%和80.0%。结论 MiR21在NSCLC患者纤维支气管镜活检组织中有较高的表达率,与病理类型和TNM分期有关,提示可能与NSCLC的发生、发展和转移有关。

Objective To investigate the expression and clinical significance of MicroRNA-21 （MiR21） in non-small cell lung cancer（NSCLC）. Methods The expression of MiR21 in the sample taken from hronchoscopy was detected in 31 NSCLC cases（group A） and 10 cancer-free cases（group C） with RT-PCR. The correlation of MiR21 expression and clinicopathological features of NSCLC was analyzed. Results The expression of MiR21 was significantly higher in group A than that in group C （P〈0.05）. The expression of MiR21 was associated with tumor classification and TNM staging. The MiR21 yielded an area under the ROlE curve （AUC） of 0. 785 （95%CI： 0. 635-0. 935） with the sensitivity of 73.3% and the specificity of 80. 0% taking the cut-off value of 0. 158. Conclusion MiR21 is highly expressed in NSCLC, which is associated with tumor classification and TNM staging, indicating that MiR21 may be related to the occurrence, development and metastasis of NSCLC.