贵州省麻疹野病毒分离株分子流行特征

An Analysis on Molecular Epidemiology Characteristics of Wild-type Measles Viral Isolation in Guizhou Province

目的:了解贵州省2008-2011年麻疹病毒(Measles Virus,MeV)分子流行病学特征,为控制和消除麻疹提供实验室依据。方法:将2008-2011年采集麻疹疑似病例的咽拭子或尿液共56份接种于Vero/SLAM(Signaling Lymphocyte Activation Molecule)细胞,培养获得8株阳性分离物,提取其病毒RNA,用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)对麻疹病毒N基因羧基末端676个核苷酸片段进行扩增,对扩增产物进行序列测定和分析。结果:分离的8株MeV均为H1基因型中的H1a基因亚型,麻疹病毒分离株之间的核苷酸相似性为97.8%～100%,氨基酸相似性为96.0%～100%,与WHO H1基因型参考株Hunan.China93-7的核苷酸和氨基酸差异分别在2.0%～2.7%和2.0%～5.3%。结论:H1a基因亚型是贵州省近年来麻疹流行的优势基因型,未发现其他基因型。

Objective： To study molecular epidemiology characteristics of wild-type measles virus （MeV） isolated in Guizhou province during 2008 - 2011, and to provide lab evidence for measles con- trol and elimination. Methods： Total 56 throat swabs and urine samples collected from suspected mea- sles cases during 2008 - 2011 were inoculated into Vero/SLAM cells, and 8 strains of positive measles isolation were successfully obtained, and viral RNA was extracted. Fragments of 676 nucleotide acids of carboxyl terminal of measles viral nucleoprotein gene were amplified by using reverse transcription- polymerase chain reaction （RT-PCR） method, and then the PCR products were sequenced and ana- lyzed. Results： All the 8 MeV strains were identified as H1 a gene subtype . The similarity of their nu- cleotide was 97.8% - 100%, and the similarity of their amino acid was 96.0% - 100%. The differ- ences of nucleotide and amino acid between 8 MeV strains and WHO H1 genotype reference strain： Hunan. China93 -7 were 2. 0% - 2. 7% and 2. 0% -5.3% respectively. Conclusions： The domi- nant genotype of wild-type measles virus is H1 a gene subtype in Guizhou province in recent years, oth- er subtype is not found.