葛根素对运动大鼠血液流变及运动能力的影响

Experimental study on effect of puerarin on hemorheology and exercise performance in trained rats

目的:分析葛根素干预对运动训练大鼠血液流变学及运动能力的影响。方法:60只8周龄SD雄性大鼠,30只口服葛根素组和30只对照组,葛根素组再分为10只葛根素安静组,20只葛根素+训练组(10只为运动后即刻组,10只为运动后恢复24h组);同样30只对照组也分10只安静组,20只训练组(10只为运动后即刻组,10只为运动后恢复24h组),建立力竭游泳训练模型。通过灌服葛根素干预后,观测大鼠力竭游泳时间,测试各组大鼠全血黏度、血浆黏度、红细胞聚集指数、红细胞刚性指数、红细胞压积、红细胞沉降率(血沉)。结果:①安静时:与对照组相比,葛根素组大鼠全血黏度显著降低[(8.41±0.76),(6.51±0.92),P<0.05],红细胞刚性指数显著降低[(1.23±0.76),(0.98±0.72),P<0.05]和红细胞聚集指数显著降低[(16.65±2.45),(14.78±1.55),P<0.05];②运动后即刻时:与对照训练组相比,葛根素训练组全血黏度显著下降(P<0.05),血浆黏度显著降低[(1.75±3.3),(1.58±0.21),P<0.05],红细胞聚集指数显著下降[(21.37±4.78),(19.43±5.86),P<0.05],红细胞刚性指数显著下降[(1.58±0.90),(1.39±0.99),P<0.05],红细胞压积显著降低[(0.71±0.45),(0.57±0.43),P<0.05];③运动后恢复24h:与对照训练组相比,全血黏度、红细胞聚集指数、红细胞刚性指数、红细胞压积显著降低(P<0.05),血浆黏度差异无显著性差异[(1.45±0.47),(1.52±0.26),P>0.05]④游泳至力竭时间:葛根素组游泳时间较对照组有显著延长(P<O.05),力竭延缓率达到28%。结论:葛根素可显著改善运动大鼠血液流变性的功能,使大鼠运动能力有了明显提高。

Objective： To observe the effects of intervention of puerarin on blood hemorheology and exercise performance of rats after exercise. Method：The 60 male SD rats aged 8 weeks were selected,randomly divided two big groups： puerarin supplement groups and control groups, each group had 30 rats .each big group divided three small groups： the sedentary group, the train immediately group and the train after 24 hours recovery group, each group had 10 rats. Then established the rats exhaust-swimming train model. After puerarin supplement the exhausting-swimming time was recorded, rats＇ exercise performances were observed and the blood viscosity, plasma viscosity, aggregability of red blood celI（ARBC）, hematocrit（HCT）,erythroeyte index of rigidity（EIR）,erythrocyte sedimentation rate（ESR）were mea- sured. Result： ①On sedentary time： Compared with control group , the whole blood viscosity of puerarin group declined significantly[（8.41 ± 0.76）,（6.51 ±0.92）, P〈0.05], the EIR declined significantly[（1.23 ±0.76）,（0.98 ± 0.72）, P〈0.05], the ARBC of puerarin group declined significantly[（16.65±2.45）,（14.78 ± 1.55）,P〈0.05];②On the train immediately： Compared with control group, the blood viscosity of puerarin group declined significantly（P〈0.05）, the plasma viscosity declined significantly[（1.75± 3.3）, （1.58± 0.21）,P〈0.05], the ARBC of puerarin group declined significantly[（21.37 ± 4.78）, （19.43 ± 5.86）, P〈0.05], the EIR declined significantly [（1.58 ± 0.90）,（1.39 ± 0.99）, P〈0.05], the HCT of puerarin group declined significantly[（0.71±0.45）,（0.57 ± 0.43）,P〈0.05]; ③After 24 hours recovery： Compared with control group, the blood viscosity, ARBC, EIR, HCT of puerarin group declined significantly（P〈 0.05）, the plasma viscosity of puerarin group had no significant difference[（1.45± 0.47）, （1.52 ± 0.26）,P〉0.05];④ The exhausted swimming time： The swimming time of the puerarin group was longer than the control group（P〈 0.05）,and the Exhaustive Delay Rate Increased to 28%. Conclusion： Puerarin could effectively prolong the exhausted swimming time and improve hemorheological indices.