食管黏膜树突状细胞表型分析

Isolation and characterization of dendritic cells from normal human esophageal mucosa

目的:研究食管黏膜树突状细胞(DCs)表型特点。方法:内镜下收集健康人食管黏膜标本,Ficoll-Hypaque密度梯度离心法分离食管黏膜标本中的单个核细胞,采用磁珠分选技术分离DCs,流式细胞仪分析食管黏膜DCs表型。结果:健康人食管黏膜含3类单个核细胞:HLA-DRhigh/CD13low,HLA-DRmed/CD13+和HLA-DR-/CD13+;HLA-DRhigh/CD13low细胞表达不同DCs亚群表面标志,是食管黏膜DCs;健康人食管黏膜DCsCD80、CD83、CD86表达水平低,是不成熟DCs。结论:成功分离了食管黏膜DCs,证实HLA-DRhigh/CD13low细胞是食管黏膜DCs。

AIM： To investigate the characteristics of dendritic cells （DCs） from normal human esophageal mucosa. METHODS： We collected normal esophageal mucosa samples from healthy individuals using endoscope and isolated mononuclear cells from the samples using FicolI-Hypaque density gradient centrifugation. DCs were further separated by magnetic-activated cell sorting （MACS） to analyze DCs subsets by flow cytometry. RESULTS： Three mononuclear cell populations were detected in normal human esophageal mucosa： HLA-DR^high/CD^13low, HLA-DR^med/CD13^＋ and HLA-DR-/CD13^＋. HLA-DR^high/CD13^low cells expressed the DCs-associated molecules and were referred to as esophageal mucosal DCs. The DCs were immature since they expressed low levels of CD80, CD83 and CD86. CONCLUSION： HLA-DR^high/CD13^low cells isolated from the normal human esophageal mucosa were proved to be DCs. DCs can be successfully isolated from esophageal mucosa.