血清P53蛋白检测对职业性肺癌的预报价值

The predication value of the detection of serum level of P53 protein in occupational lung cancer

目的 :探讨血清P5 3蛋白作为职业性肺癌早期监测指标的可能性和应用价值。方法 :对长期接触多环芳烃混合物 (PAHs)的 2 3名焦炉工和 18名沥青工进行外周血淋巴细胞染色体畸变检查初筛 ,检出染色体畸变者各16名 ,然后采用双抗夹心酶联免疫吸附试验方法 (Sandwich ELISA)测定畸变者血清P5 3蛋白水平。以 12名正常献血员为对照组。结果 :焦炉工和沥青工血清P5 3水平均值高于对照组 (P <0 .0 5 ) ,其中 10例高于对照组 ( x +2s)。结论 :血清P5 3水平可从基因水平反映PAHs对机体遗传物质的损伤作用 ,该指标有可能成为职业性肺癌早期监测的又一途径。

Aim:Discuss the application value and possibility of serum level of P53 protein as a early monitoring marker to the occupational lung cancer.Method:23 coke workers and 18 pitch workers were chosen as the exposure group,and the control group was composed of 15 non exposed and healthy men.They were primarily assayed by the peripheral lymphocyte chromosomal aberration (CA).And 16 coke workers and 16 pitch workers and 12 men of the control were tested for serum level of P53 after reviewing the CA results. According to the method of the culture of peripheral lymphocyte and preparation of chromosome sample,the examination of CA was carried out. Serum level of P53 was determined by Sandwich ELISA. Results: Serum level of P53 of exposed group was higher than that of control group ( P <0.05).10 of the exposed group were determined as positive because their P53 level were greater than 2SD above the control average. Conclusion: The results showed that P53 protein was detectable in a proportion of coke and pitch workers,the serum level of P53 can reflect genetic injury of long term exposure to PAHs. And thus,as a monitoring marker for early cancer, the detection of serum level of P53 has certain applicant value.Moreover, co application of many genetic toxicologic effect markers, such as CA, will be an effective way to improve prediction of cancer.