黄绿青霉素致大鼠心肌损伤的实验观察

Experimental study on the myocardium damages in duced by CIT in rats

目的 观察黄绿青霉素 (citreoviridin,CIT)对大鼠心肌的损伤。方法 用含 CIT(每日每 kg体重15 m g)的染毒饲料喂养大鼠 1个月 ,取心肌和肝脏组织 ,石蜡切片 ,光镜观察。结果 实验组大鼠心肌在左室壁内膜下、心尖、室中隔部位出现明显坏死等改变 ,病变呈灶状或条索状分布 ,涉及范围较大 ,有淋巴细胞和单核细胞浸润 ,心肌细胞呈颗粒变性 ,肌原纤维凝聚、崩解 ,部分肌纤维溶解。结论 CIT可致大鼠心肌原发性坏死 ,好发部位在左室内膜下 。

Objective Observe the effects of CIT on the myocardium in rats with optical microscope.Methods The rats were fed with contaminated feed in the dose of CIT 15mg/kg body for one month,the heart and liver were taken away and embedded with parafine.We observed the sections with optical microscope.Results In the subendocardium of the left ventricle,apical part and interventricular septum,the pathologic changes of degeneration and necrosis were found out in some bigger size of point or rope type separately,in which the filtration of lymphocyte and mononuclear cells were seen in great quantities.The myocardial cells presented granular denaturation and myofibril lysis.Conclusions CIT caused original necrosis changes distributing mostly in the subendocardium of left ventricle,interventricular septum and apical tissue. [