人肺癌组织细胞Na^+/H^+交换蛋白-1基因的表达与其抗凋亡的关系

Expression of Na^+/H^+ Exchanger-1 gene in human lung cancer tissue cells and its role in anti apoptosis

目的 :通过对临床人肺癌组织Na+/H+交换蛋白 1(NHE 1)mRNA的表达的检测 ,探讨肿瘤治疗新的特异靶点。方法 :采用RNA原位分子杂交法 (RNA IMH)检测了 2 7例人肺癌组织和正常肺组织NHE 1mRNA的表达。结果 :人肺癌组织细胞NHE 1mRNA的表达显著增强 ,呈过表达 ,而各病理类型间无显著差异。结论 :人肺癌组织细胞中NHE 1mRNA过表达这一区别于正常组织细胞的分子生物学特征与肿瘤细胞的抗凋亡特性的形成密切相关 。

Purpose:To explore a new and specific target of anticancer strategy by the measurement of expression of Na +/H + exchanger 1(NHE 1) mRNA in human lung cancer tissue. Methods:The expression of NHE 1 mRNA in human lung cancer tissues as well as normal lung tissues was measured with the method of in situ RNA molecular hybridization (RNA IMH). Results: It was found that NHE 1 mRNA expression in human lung cancer tissue cells was significantly enhanced, while there was no significant difference among various pathological entities of lung cancer. Conclusions:There is overexpression of NHE 1 mRNA in human lung cancer tissue cells, which is an important mechanism maintaining tumor cell growth characteristce of anti apoptosis by preventing intracellular acidification. So there may be an appropriate and specific target as a molecular biological feature of tumor cells different from that of normal cells,which may be of potential clinical significance.