高压氧治疗对颅脑损伤大鼠谷胱甘肽过氧化物酶及丙二醛的影响

The effect of hyperbaric oxygen on plasma levels of glutathione peroxidase and maleic dialdehyde after traumatic brain injury in rats

目的观察高压氧(hyperbaric oxygen,HBO)治疗对创伤性颅脑损伤(traumatic brain injury,TBI)模型大鼠氧化-抗氧化指标的影响,为临床TBI患者实施HBO治疗提供基础实验依据。方法雄性Wistar大鼠分为脑损伤组、损伤后HBO治疗组、空白对照组。采用侧位液压撞击(lateral fluid percussion,LFP)法建立TBI大鼠模型。分光光度法测量血清中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力、丙二醛(maleic dialdehyde,MDA)含量。结果 HBO治疗组GSH-Px活力显著高于损伤组(P<0.01),MDA含量两组差异无统计学意义(P>0.01)。HBO治疗各组GSH-Px活力均高于相应脑损伤组,亚急性期开始行HBO,治疗组GSH-Px活力显著高于相应脑损伤组(P<0.01)。结论在TBI模型大鼠损伤后特定时间段内给予HBO治疗,可使模型大鼠抗氧化能力短时间内迅速提高,所以适时适量的HBO治疗,不但不增加氧化损伤,反而减轻氧化损伤。

Objective To study the effect of hyperbaric oxygen （HBO） therapy on oxidation and antioxidation in traumatic brain injury （TBI） rat model and provide basical data for the clinic application. Methods Wistar rats were randomly divid- ed into three groups： TBI group, TBI＋HBO treatment group, blank control group. TBI models were established by exposing to a lateral fluid percussion （LFP）. The activity of glutathione peroxidase （GSH-Px） and the concentration of maleic dialde- hyde （MDA） were detected by spectrophotometry. Results The activity of GSH-Px in TBI＋HBO group was higher than that of TBI group （P 〈 0.01）. The level of MDA had no statistical difference （P 〉 0.05） between the TBI＋HBO group and TBI group. The activity of GSH-Px was higher in each group with HBO treatment than those groups without HBO treatment. HBO treatment during subacute phase improved the activity of GSH-Px significantly （P 〈 0.01）. Conclusion The adminis- tration of HBO therapy during early stage can enhance antioxidant abilities in TBI rat model. A proper HBO therapy may reduce the oxidative damage rather than increase the oxidative damage.