HBV感染者免疫调节细胞因子表达变化的研究

Study on the variation of immunological cytokines expression in HBV infected person

目的研究慢性乙型肝炎病毒（HBV）感染者免疫状况以及免疫调节细胞因子含量变化情况,了解病毒载量、肝功能与IFN-γ、IL-2、IL-4、IL-10、IL-12等细胞因子含量变化情况的临床意义。方法收集27例HBeAg阳性和肝功能ALT异常者为HBV感染现症患者为A组;22例HBeAg阳性和肝功能ALT持续正常者为无症状HBV携带者为B组;11例HBeAg阴性和肝功能ALT正常以及HBV-DNA阴性者为非活动性HBsAg携带者为C组;设正常对照组。采用双抗体夹心ELISA法定量检测IFN-γ、IL-2、IL-4、IL-10、IL-12血清含量。结果 HBV感染者各组IL-2、IL-12含量较正常对照组均下降,IL-4、IL-10均升高,差异有统计学意义（P〈0.05）,IFN-γ普遍降低但各组之间差异无统计学意义（P〉0.05）。A组现症患者IL-2、IL-12的活性显著高于B组无症状HBV携带组和C组非活动性HBsAg携带组（P〈0.05）;IL-4、IL-10含量各组均低于正常对照组,差异有统计学意义（P〈0.05）,患者各组间差异无统计学意义（P〉0.05）。病毒载量与细胞因子含量的关系：IFN-γ、IL-2各组与正常对照组比较显著下降（P〈0.05）,IFN-γ下降幅度为≥107组和105~6组〉、〈105组,差异有统计学意义（P〈0.05）。IL-12也普遍降低,各组差异无统计学意义（P〉0.05）。IL-4、IL-10含量各组明显高于对照组（P〈0.05）,含量随病毒载量增高而增加。中、高病毒载量组与低病毒载量组差异有统计学意义（P〈0.05）,低病毒载量组与正常对照组差异无统计学意义（P〉0.05）。结论 HBV感染状态和病毒量与免疫调节细胞因子IL-2、IL-12、IL-4、IL-10含量变化有一定的关联性。

Objective To study the immune condition and the concentration changes on immunological cytokines in HBV infected person, to find out the clinical significances of virus loads, liver function and some cytokincs concentration changes such as IFN-γ IL-2, IL-4, IL-10, IL-12 and so on. Methods 27 patient cases with the HBeAg^＋ and ALT func- tion of liver abnormally was group A; 22 asymptomatic HBV carrier cases with HBeAg＋ and ALT function keeping normally was group B; Group C was 11 in-activity HBeAg cartier cases with the HBeAg-,ALT function normally and HBV-DNA negatively. The forth group was normal control, quantitative detected the concentration changes of IFN-γ, IL-2, IL-4, IL- 10, IL-12 in serum with the double -antibody sandwich methods. Results The concentration of IL-2, IL-12 in HBV in- fected person in every group decreased and the concentration of IL-4, IL-10increased compared with the normal control group, the difference was significant （P 〈 0.05）; the concentration of IFN-γ commonly decreased but the difference was not significant （P 〉 0.05）; the activity of IL-2, IL-12 in group A was significantly higher than group B and group C （P 〈 0.05）; the concentration of IL-4, IL-10 in every group was lower than normal control group, the difference was significant （P 〈 0.05）. The difference between patient groups had no statistical significance. The relations between virus loads and cytokines： IFN-γ and IL-2 in every group significantly decreased compared with the normal control group （P 〈 0.05）, the width of the IFN-γdeclining was ≥ 10^7 group and 10^5-6 group 〉, 〈10^5 group, the differences was significantly （P 〈 0.05）, IL-12 decreased commonly and the difference between every groups had no significance （P 〉 0.05）. The concentra- tion of IL-4, IL-10 in every group was obviously higher than normal control group （P 〈 0.05）, it increased along with the concentration of virus loads increasing. The difference between the middle/high virus loads group and low virus loads group was significant （P 〈 0.05）, the difference between low virus load group and the normal control group was not significant （P 〉 0.05）. Conclusion The infection status and the virus quantity of HBV correlated with the content variation of the im- munoregulatory cytokines IL-2, IL-12, IL-4, IL-10.