慢性乙型肝炎免疫清除期患者HBsAg基因多态性研究

Study on polymorphism of hepatitis B virus surface antigen in immune clearance stage in patients with chronic HBV infection

目的探讨慢性乙型肝炎免疫清除期患者乙型肝炎病毒表面抗原（HBsAg）基因多态性。以寻找与乙型肝炎病毒免疫清除相关的变异位点。方法回顾性分析我院2008年2月~2011年12月感染性疾病科门诊及住院的慢性乙型肝炎免疫清除期患者10例,并设计特异性引物,于10例患者血清中扩增S基因片段,TA克隆法克隆到T载体中,随机选择克隆测序。结果共20个克隆被测序,20个克隆S蛋白发现12个不同位点的32次变异。主要集中在T细胞表位,B细胞表位及a决定簇。结论慢性乙型肝炎免疫清除期患者存在HBsAg变异,可能仍然存在HBsAg免疫耐受。

Objective To study the amino acid sequence polymorphism of hepatitis B virus （HBV） surface antigen in immune clearance stage in the patients with chronic HBV infection, to find and hepatitis b virus immune clearance related variation site. Methods 10 patients with chronic hepatitis B in immune clearance phase of infectious diseases department in our hos- pital form February 2008 to December 2011 were retrospectiely analyzed, and design specific primer, serum augmentation S gene fragments were added in 10 patients, TA cloning method to cloning T carrier, and cloning sequencing was random selected. Results 20 clones of target regions were sequenced, and 32 times rariation of 12 different amino acid substations were found, located in T cell epitope, B cell epitope and the ＂a＂ determinant. Conclusion HBsAg variant is common in the patients in immune clearance stage with chronic HBV infection, it is likely existing HBsAg immune tolerance in those pa- tients.