子宫内膜异位症患者血清及腹腔液6-异前列腺素F1α的表达及意义

Expression of 6-k-PGF1α in Serum and Peritoneal Fluid in Endometriosis and Its Clinical Significance

目的探讨子宫内膜异位症(内异症)患者血清及腹腔液中6-异前列腺素F1α(6-k-PGF1α)的表达及其在内异症发病中的作用。方法选择2010年6月至2012年1月在我院妇科手术治疗并经病理证实为内异症的42例患者并进行分期,其中早期(Ⅰ期、Ⅱ期)20例,晚期(Ⅲ期、Ⅳ期)22例;并选择同期无月经改变、病理证实为正常子宫内膜的子宫肌瘤患者20例。所有患者均在术前采集上肢静脉血,术中采集腹腔液。采用酶联免疫吸附(ELISA)方法测定血液和腹腔液中6-k-PGF1α的水平,并对内异症患者血清和腹腔液中6-k-PGF1α的表达进行Pearson相关性分析。结果①内异症早期组、晚期组血清、腹腔液中6-k-PGF1α水平均比对照组明显增高(均P<0.05);②内异症晚期组组血清、腹腔液中6-k-PGF1α水平均比内异症早期组明显增高(P<0.05);③内异症组血清与腹腔液6-k-PGF1α水平之间呈明显正相关(r=0.580,P=0.008)。结论内异症患者血清及腹腔液中6-k-PGF1α水平明显增高,后者可作为评判内异症严重程度的参考指标。

Objective To explore the expression of 6-k-PGF1α in serum and peritoneal fluid in endometriosis and its clinical significance. Methods 42 patients with endometriosis confirmed by pathology and gynecological surgery in our hospital from June 2010 to January 2012 were selected and divided into two groups： early stage group （stage Ⅰ and stage Ⅱ, n = 20） and late stage group （stage Ⅲ and stage Ⅳ, n = 22）. In addition, 20 uterus myoma patients with normal endometrium confirmed by pathology at the same period were selected as the control group. The serum and peritoneal fluid of all cases were collected respectively and the 6-k-PGF1α expression in serum and peritoneal fluid were detected by ELISA method. The correlation analysis between 6-k-PGF1α of serum and peritoneal fluid was performed with Pearson analysis. Results ①The 6-k-PGF1α expression in serum and peritoneal fluid in endometriosis increased significantly than control group （P 0.05）; ②The 6-k-PGF1α expression in late stage was significantly higher than early stage （P 0.05）; ③There was significant positive correlation of 6-k-PGF1α in serum and peritoneal fluid （r = 0.580, P = 0.008）. Conclusions The 6-k-PGF1α expression in serum and peritoneal fluid significantly increases in patients with endometriosis, which may be a useful marker for determining the severity of endometriosis.