碱性成纤维细胞生长因子对骨髓基质细胞粘附、增殖和分化的影响

Biological effects of basic fibroblast growth factor on bone marrow stromal cells

目的探讨碱性成纤维细胞生长因子（ｂＦＧＦ）对骨髓基质细胞（ＢＭＳＣ）粘附、增殖和分化等生物学效应的影响。方法利用体视学计数、四唑盐（ＭＴＴ）比色法及碱性磷酸酶（ＡＬＰ）试剂盒分别测定不同浓度ｂＦＧＦ诱导一定时间后ＢＭＳＣ的粘附特性、增殖和分化情况的变化。结果１０ｎｇ／ｍｌ ｂＦＧＦ明显促进ＢＭＳＣ的粘附，但是２００ｎｇ／ｍｌ ｂＦＧＦ反而不利于细胞粘附；在细胞增殖和分化测定中，１００ｎｇ／ｍｌ ｂＦＧＦ明显促进细胞增殖但细胞碱性磷酸酶含量也最低。结论碱性成纤维细胞生长因子对骨髓基质细胞的生物效应是复杂和多方面的，可以作用于骨髓基质细胞的粘附、增殖和分化等多个环节，这种影响与碱性成纤维细胞生长因子的剂量有关。

objective To investigate the biological effects of basic fibroblast growth factor(hFGF) on the adhesion, proliferation and differentiation of bone marrow stromal cells(BMSC) Methods BMSCs were subjected to the treatment with different concentrations of bFGF. after which the attached cells were estimated with stereological method. cell proliferation measured with methylthiazol tetrazolium (MTT) method and cell differentiation with alkaline phosphatase (ALP) measurement kit Results 10 ng/ml bFGF can promote cell adhension significantly. while 200 ng/ml bFGF inhibited cell adhesion. we also found that 100 ng/ml bFG F could significantly promote cell proliferation, and cellular ALP level. however. was reduced to the lowest of all groups Conclusion bFGF may have complicated and multiple biological effects on BMSC. regulating cell adhension, proliferation and differentiation Its effects are concentration dependent