乙酰胆碱诱导的离体兔心房颤动模型的建立

Establishment of acetylcholine-induced atrial fibrillation model in isolated rabbit heart

目的建立乙酰胆碱诱导的离体Langendorff灌流的兔的心房颤动模型。方法健康雄性的新西兰兔40只,2.2～2.5kg分为4组,分别为正常灌流组、正常灌流+burst刺激组、正常灌流+乙酰胆碱灌注组和正常灌流+乙酰胆碱灌注+burst刺激组,每组各10只。在Langendorff灌流装置下行主动脉逆行灌流,通过乙酰胆碱灌注和burst刺激诱发心房颤动。结果正常灌流组、正常灌流+burst刺激组和正常灌流+乙酰胆碱灌注组均没有发生心房颤动,而正常灌流+乙酰胆碱灌注+burst刺激组心房颤动的发生率为50%。结论在Langendorff心脏灌流时,给予乙酰胆碱灌注模拟胆碱能激活,并给予burst刺激模拟应激事件,可以稳定地诱导心房颤动的发生。

Objective To establish an acetylcholine-induced atrial fibrillation （AF） model in the isolated rabbit heart. Methods Forty healthy male New Zealand rabbits （2.2 -2.5 kg） were used in the study. The isolated rabbit hearts were randomly assigned to normal perfusion （group A）, normal perfusion with burst high-frequency electric stimulation （group B ）, acetylcholine perfusion （group C） and acetylcholine perfusion with burst electric stimulation （group D） （n = 10 in each group）. The isolated hearts were perfused with Langendorff perfusion system. Results The AF incidence in group D （acetylcholine perfusion combined with burst electric stimulation） was 50% ;while no AF was induced in other three groups. Conclusion A simple and stable acetylcholine-induced atrial fibrillation model has been established in isolated rabbit heart with Langendorff perfusion system.