载转化生长因子-β_1壳聚糖-聚己内酯微球制备及其可控释放性质研究

Preparation of Chitosan-Polycaprolactone Microspheres for Controllable Delivery of Transforming Growth Factor-Beta1

目的:制备载转化生长因子β1(TGF-β1)的壳聚糖-聚己内酯微球并研究其体外缓释行为。方法:以三聚磷酸钠为交联剂,利用水/油乳液方法制备载TGF-β1因子的壳聚糖-聚己内酯微球。利用酶联免疫吸附方法(ELISA)检测壳聚糖-聚己内酯微球的体外缓释行为。结果:在目前制备条件下,壳聚糖-聚己内酯微球具有好的形态,平均粒径可有效控制在10-20μm范围内。壳聚糖-聚己内酯中的聚己内酯含量能有效控制微球的初始释放和随后的缓释行为。优化获得的壳聚糖-聚己内酯微球能够以不同的速率控制TGF-β1持续释放超过4周。结论:载TGF-β1因子壳聚糖-聚己内酯微球的制备工艺稳定和可控,其初始释放和持续缓释行为可以由壳聚糖-聚己内酯中聚己内酯的含量有效调控。

Objective： TGF-β1-loaded chitosan-polycaprolctone（CH-PCL） microspheres were prepared and their TGF-β1 release behavior was examined.Methods： TGF-β1-loaded CH-PCL microspheres were produced by an emulsion method using sodium tripolyphosphate as a crosslinker.The release patterns of TGF-β1 inside the microspheres were investigated using enzyme-linked immunosorbent assay（ELISA）.Results： CH-PCL microspheres showed a good sphericity with various average diameters changing between 10 and 20 μm.The percentage of PCL inside CH-PCLs could effectively control the release behavior of the microspheres at the early stage as well as the follow-up duration.Some optimal microspheres were capable of administrating the release of TGF-β1 in a well-controlled manner over four weeks with greatly reduced initial burst.Conclusion： The presented method for preparing TGF-β1-loaded CH-PCL microspheres was controllable and stable,and meanwhile,the release behavior of the microspheres could also be effectively modulated by the PCL content in CH-PCL.