人巨细胞病毒感染与染色体着丝粒点变异关系研究

A study on the relationship between the infection of human cytomegalovirus(HCMV) and the variation of kinetochore

目的 :研究孕妇HCMV感染对胚胎染色体着丝粒点 (Cd)变异的影响 ,探讨HCMV感染引起出生缺陷的原因和机制。方法 :用我室改良的Cd -NOR同步银染技术对 15例HCMV感染和 2 5例非感染孕妇的胚胎或绒毛组织Cd消失、Cd变异进行比较分析。结果 :前者Cd消失、Cd变异频率较后者有显著和极显著的增高。结论 :染色体Cd的变化可能是HCMV感染引起胚胎发育异常的重要原因之一 。

Objective:We studied the relationship between the infection of HCMV and the variation of kinetochore , researched the mechanism of causing abnormal growth embryo. Methods:Kinetochore (centromeric dots,Cd) of chorionic villus or embryo tissue of 15 HCMV-DNA(PCR) positive and 25 HCMV-DNA(PCR) negative pregnant women were studied by Cd-banding techniques. Results:The frequency of Cd loss and Cd size variation in positive group were significantly higher than those in negative group. Conclusion:The study indicated that Cd variation caused by HCMV infection play an important role in form of birth defect. Cd banding analysis in chorionic villus or embryo tissue of HCMV-DNA(PCR) positive pregnant women could evaluate the risk of abnormal growth embryo.