Dot-ELISA法检测家蚕核型多角体病毒蛋白组份方法的建立及应用

Establishment and use of a Dot-ELISA for determination of the protein component of bombyx mori nuclearpolyhedrosis virus

目的建立家蚕核型多角体病毒蛋白的 dot- EL ISA检测方法。方法免疫家兔制备抗蚕核型多角体病毒蛋白组份的多克隆抗体 ,并用 EL ISA间接法建立了检测方法。结果此法与人血清和兔血清无交叉反应 ,可检测微量多角体病毒蛋白 ,其灵敏度达 10 ng,且操作简便。

ObjectiveIn order to establish an indirect type of dotELISA for the protein of Bombyx mori nuclear polyhedrosis virusMethod Polyclonal antibody to the protein of BmNPV was produced by conventional immunization to rabbit,the assay was established according to indirect type of ELISAResults No cross reaction was found with human serum and rabbit serum,the sensitivity of the immunoassay was 10ngConclusion This assay could be applied in detecting the leftover protein of the product that BmNPV gene expression system produced and the serum of infected mice [