人DAO氨基酸序列片段B-细胞表位的多参数预测

The multi-parameter prediction of B-cell epitopes in amino acid sequence fragment of human DAO

目的预测人二氨氧化酶 ( DAO)氨基酸序列片段 ( aa No.5 2 4- 6 18)的 B-细胞表位。方法应用 6种参数和方法进行综合分析 ,包括 Hopp & Woods亲水性参数、抗原性参数、可及性参数、β-转角、万氏及吴氏综合预测方法。结果显示 B-细胞识别的表位可能在 5 43- 5 5 3和 5 6 9- 5 95残基或其附近 ,5 6 9- 5 95残基 4种参数和方法的预测值均高于 5 43- 5 5 3残基 ,这两个被预测的表位均含有β-转角和不规则卷曲结构。结论本研究为应用合成肽抗原制备抗人

ObjectiveTo predicate Bcell epitops in amino acide sequence (aaNo524618) of human diamine oxidase(DAO)Method Six parameters and methods were used,including Hopp & Woods hydrophility,antigenicity(Welling),accessibility(Janin),βturns,Wans method and Wus methodResults It showed that Bcell epitops were probably at or adjacent to 543553 and 569595The predicted values of 4 parameters and methods of 569595 was higher than 543553All the 2 predicted epitops are rich in βturns and random coilConclusionThe finding is helpful for preparation of antibody to human DAO using the synthetic peptide approach [