细胞外基质金属蛋白酶诱导因子和尿激酶型纤溶酶原激活物在载脂蛋白E基因敲除小鼠主动脉粥样硬化斑块中的表达

Expression of Extracellular Matrix Metalloproteinase Inducer and Urokinase-Type Plasminogen Activator in Aortic Atherosclerotic Plaques of ApoE Knockout Mice

目的探讨细胞外基质金属蛋白酶诱导因子(EMMPRIN)与尿激酶型纤溶酶原激活物(uPA)在载脂蛋白E基因敲除(ApoE-/-)小鼠主动脉粥样硬化斑块中的表达,并探讨两者的相互关系。方法研究对象采用ApoE-/-小鼠,共喂养18周,分别于6周、10周、14周和18周4个时间点处死小鼠行HE染色切片观察动脉粥样硬化斑块形态;并采用RT-PCR及Western blot检测主动脉粥样硬化斑块内EMMPRIN和uPA的表达。结果成功建立动脉粥样硬化模型;在主动脉粥样硬化斑块中检测到EMMPRIN和uPA表达均增高,与普通饮食组比较有统计学意义(P<0.05)。EMMPRIN和uPA表达随喂养时间延长而增高。结论 EMMPRIN和uPA在ApoE-/-小鼠主动脉粥样硬化斑块中随着斑块病变程度的严重性增加表达增高,两者可能共同参与粥样硬化斑块的发生发展过程。

Aim To investigate the expression of extracellular matrix metalloproteinase inducer（EMMPRIN） and urokinase-type plasminogen activator（uPA） in aortic atherosclerotic plaques of apolipoprotein E knockout（ApoE-/-） mice and to explore the relationship between the two.Methods ApoE-/-mice were randomized into ordinary diet fed group and high-fat diet fed group,raising for 18 weeks.At 6 weeks,10 weeks,14 weeks,18 weeks mice were sacrificed and observed by HE staining of atherosclerotic plaque morphology.EMMPRIN and uPA expression in aortic atherosclerotic plaques were detected by semi-quantity RT-PCR and Western blot.Rusults Atherosclerosis model was successfully established;EMMPRIN and uPA in aorta atherosclerotic plaques were highly expressed,with statistically significant difference from normal diet group（P〈0.05）.Expression of EMMPRIN and uPA increased with feeding time extended.Conclusion Expression of EMMPRIN and uPA in ApoE-/-mice aortic atherosclerosis increased with plaque lesions degree of severity, both ofEMMPRIN and uPA may play a role in the atherosclerotic plaques development.