胆固醇酯转运蛋白TaqIB基因多态性与颈动脉粥样硬化斑块稳定性的关系

Relationship of Cholesterol Ester Transfer Protein TaqIB Polymorphism with the Stability of Carotid Atherosclerosis

目的探讨胆固醇酯转运蛋白(CETP)TaqIB基因多态性与脑梗死患者颈动脉粥样硬化斑块稳定性之间的关系。方法对135例颈动脉易损斑块的脑梗死患者和158例颈动脉稳定斑块的脑梗死患者进行研究。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法测定所有研究对象血标本的CETP TaqIB基因多态性,对易损斑块组和稳定斑块组CETP TaqIB基因多态性进行比较分析。结果易损斑块组B1B1、B1B2、B2B2基因型比例分别是56.30%、33.33%和10.37%,稳定斑块组分别为53.80%、36.07%和10.13%,两者比较差异无统计学意义。B1、B2等位基因在易损斑块组的频率分别为72.96%及27.04%,在稳定斑块组为71.84%及28.16%,两者差异也无统计学意义。结论胆固醇酯转运蛋白TaqIB基因多态性与脑梗死患者的颈动脉粥样硬化斑块稳定性没有明确的关系。

Aim To explore the relation between the genetic polymorphism cholesterol ester transfer protein （CETP） TaqIB and the stability of carotid atherosclerosis of cerebral infarction patients. Methods 135 cases of cere- bral infarction with vulnerable plaques and 158 cases of cerebral infarction with stable plaques were studied. Polymerase chain reaction - restriction fragment length polymorphism （PCR-RFLP） method was adopted to determine CETP TaqIB gene polymorphism for all specimens, comparing CETP TaqIB gene polymorphism in the vulnerable plaque group with the stable plaque group, which were statistically analysed. Results The proportions of B1 B1, B1B2, B2B2 genotype in the vul- nerable plaque group were 56. 30%, 33.33% and 10. 37% respectively, and were 53.80%, 36. 07% and 10. 13% in the stable plaque group, the difference has no statistical significance. The frequencies of B1, B2 allele in the vulnerable plaque group were 72. 96% and 27.04% respectively, and were 71.84% and 28. 16% in the stable plaque group, the difference also has no statistical significance in comparison. Conclusions CETP TaqIB genetic polymorphism may have no definite relationship with the stability of carotid atherosclerosis of cerebral infarction patients.