维泰醇对小鼠宫颈癌U_(14)细胞抗肿瘤作用的研究

Effect of Alternol on the anti-cancer of mouse cervical carcinoma U_(14) cells

目的:研究维泰醇对宫颈癌U14细胞的抑制增殖与诱导凋亡作用,以探讨维泰醇的抗肿瘤作用机制。方法:采用MTT法检测维泰醇对宫颈癌U14细胞增殖抑制率的影响;采用AO/EB双染色法观察细胞凋亡形态并计算其凋亡率;免疫细胞化学法检测U14细胞内Bcl-2,Bax、Survivin蛋白表达水平。结果:维泰醇对U14细胞增殖有明显的抑制作用,呈剂量及时间依赖性;维泰醇能引起细胞凋亡,并出现典型的凋亡形态。维泰醇下调U14细胞内Bcl-2、Survivin蛋白表达水平,上调Bax蛋白表达水平。结论:维泰醇可抑制宫颈癌U14细胞增殖并诱导细胞凋亡;维泰醇抗宫颈癌的作用机制可能与其下调Bcl-2、Survivin蛋白的表达及上调Bax蛋白的表达有关。

Objective：To investigate the effect of Ahernol on the proliferation and apoptosis of mouse cervical car- cinoma U14 cells and explore its anti - cancer mechanism. Methods：The inhibitory effect of Alternol on U14 cells was measured by MTF assay. The morphology of apoptotic cells and the apoptotic ratio were observed by acridine orange/ ethidium bromide （AO/EB） double staining. The expressions of Bel -2, Bax and Survivin proteins were observed by immunocytochemistry. Results： Ahernol inhibited the proliferation of U14 cells in a dose - and time - dependent man- ner significantly. Ahernol induced apoptosis of U14 cells conspicuously, and appear typical morphology of apoptotic. Ahernol could down - regulate the expressions of Bcl - 2 and Survivin proteins, and up - regulate the expression of Bax proteins. Conclusion：Ahernol inhibits the proliferation and induce apoptosis of cervical carcinoma Ul4 cells ef- fectively. Alternol down - regulated expression of Bcl - 2 and Survivin proteins, and up - regulated expression of Bax proteins maybe related to the mechanisms of anti - cervical carcinoma.