荧光偏振法检测高危HPV16型E6基因350位(T→G)突变

Detection of high-risk HPV16 E6 gene nt350(T→G) point mutation in cervical cancerous tissue by using TDI-FP technique

目的:检测宫颈癌人类乳头瘤病毒(HPV)16 E6基因350位(T→G)点突变情况。方法:应用TDI-FP方法检测宫颈癌患者HPV16E6基因350位(T→G)点突变情况。TDI-FP技术是一种在聚合酶链反应的基础上具备液相探针杂交和碱基掺入三重特异反应的检测新方法,具有极高的特异性和敏感性。结果:应用TDI-FP法检测宫颈癌组织中HPV16型E6基因350位(T→G)突变率为6.06%,HPV16 E6基因350位突变与对照组相比无统计学差异(P=0.603,P>0.05)。结论:本地区宫颈癌患者中HPV16型E6 350位(T→G)很少发生突变,有别于欧洲的HPV16型E6 350位(T→G)高突变。

Objective：To detect HPV16 E6 nt350 （T→G） point mutation in cervical cancerous tissue. Methods： We detected the point mutation of HPV16 E6 nt350（T→G） in HPV 16 positive cervical cancerous tissue by TDI- FP method. TDI - FP technique is a compounded reaction including PCR, probe hybridization and base incorporation with high specificity and sensitivity. Resuits：HPV16 E6 nt350 （T→G）point mutation rate was 6.06%. There was no significant difference of HPV 16 E6 nt350 （T→G）point mutation rate between the cervical cancer group and control group （ P 〉 0.05 ）. Conclusion： It can be identified that the point mutation of HPV 16 E6 nt350 （T→G） seldom oc- cur, which mutation rate is very low.