紫外线照射对人晶状体上皮细胞内ALDH1蛋白表达的影响

Effect of ultraviolet radiation on ALDH1 expression in human lens epithelial cells

目的:通过对紫外线(ultraviolet,UV)照射后人晶状体上皮细胞(human lens epithelial cell,HLEC)ALDH1蛋白表达变化的观察,探讨UV诱导HLEC凋亡的可能机制。方法:以实验室培养的HLEC细胞株为研究模型,采用同一UV光源对HLEC进行照射。按UV照射时间将HLEC分为0,5,10,15及30 min组,用AO/EB染色法检测细胞凋亡,用免疫组织化学的方法观察ALDH1在各组的表达,用Western印迹对其蛋白含量进行测定。结果:ALDH1免疫组织化学染色阳性细胞率随着UV照射时间延长而降低(P<0.05),与HLEC凋亡率间呈负相关(r=–0.92,P<0.05);Western印迹灰度值随UV照射时间延长逐渐下降(P<0.05)。结论:HLEC内ALDH1含量随UV照射时间及剂量增加而下降,这可能与UV导致的HLEC凋亡相关。

To determine the apoptosis-inducing effect of ultraviolet light （UV） on human lens epithelial cell （HLEC） and to explore the involvement of changes in ALDH1 folowing UV radiation. Methods： HLEC was exposed to the same UV light source and was subsequently divided into 6 groups according to UV radiation time of 0 （control group）, 5, 10, 15, and 30 min. Apoptosis was detected by AO/EB staining. Changes of ALDH1 in HLEC were detected by immunohistochemical staining and Western blot. Results： The intensity of immunohistochemical staining and the rate of positive cells decreased with increase of UV time （P〈0.05）. The rate of positive ALDH1 cells was negatively correlated with the rate of apoptosis （r= -0.92, P〈0.05）. Western blot showed the integrated absorbance values significantly decreased with the increase of UV time （P〈0.05）. Conclusion： ALDH1 in HLEC decreases with an increase of UV exposure, which may be related to UV induced apoptosis of HLEC.