脂联素基因多态性与代谢综合征相关性研究

Association of adiponectin gene polymorphism with metabolic syndrome

目的分析脂联素基因(apM1)多态性与代谢综合征的相关性。方法无亲缘关系的344例汉族不同程度代谢异常者,根据代谢异常程度分为代谢综合征组180例(MS组)与代谢异常组164例,选择同期50例无代谢异常者为对照组,采用多聚酶链反应-限制性片段长度多态性方法检测3组apM1基因外显子2+45和外显子3+331 2个位点的基因多态性,并检测血脂等指标。结果中国汉族人apM1基因外显子2+45位点存在基因多态性,有TT,TG,GG 3种基因型;3组间基因型频率及等位基因频率比较差异无统计学意义(P>0.05);G等位基因携带者(G+)与非G等位基因携带者(G-)血清脂联素、血脂、血糖及胰岛素水平差异无统计学意义(P>0.05);中国汉族人外显子3+331位点不存在基因多态性,均为TT基因型。结论中国汉族人脂联素基因外显子2+45位点的多态性与代谢综合征无相关性;外显子3+331位点不存在基因多态性。

Objective To investigate the association of adiponectin gene （apM1） polymorphism with metabolic syndrome. Methods A total of 344 unrelated subjects were divided into metabolic syndrome group （n = 180） and abnormal metabolism group （n= 164） according to the degree of abnormal metabolism. Another 50 cases of normal metabolism were as controls. All these three groups were detected apM1 exon 2＋45T→G and the apM1 exon 3＋331T→ C with PCR RFLP technique. Results The polymorphisms existed at nucleotide ＋ 45 in exon 2 region in apM1 gene in Chinese Han people, and two alleles of T and G existed, consisting of three genotypes： TT, TG and GG. There were no significant differences in genotypic frequency and allele gene frequency among these groups （P〉0.05）. There were no significant differences in the clinical characteristics, such as the concentrations of serum adiponectin, glucose and insulin between the subjects with allele gene G and the subjects without allele gene G （P〉0.05）. There were no polymorphisms at nucleotide ＋331 in exon 3 region in apM1 gene in Chinese Han people, and the genotype was TT. Conclusion In Chinese Han people, the exon 2＋45 polymorphism in apM1 is not correlated with metabolic syndrome, and the exon 3＋ 331 polymorphism in apM1 gene does not exist.