静脉血栓栓塞症患者外周血单个核细胞T细胞免疫功能相关基因mRNA表达

The expression of T cell immune-related gene mRNAs in peripheral blood mononuclear cells from patients with venous thromboembolism

目的探讨静脉血栓栓塞症（VTE）患者T细胞免疫功能在VTE发病中的作用。方法采用全人类基因表达谱芯片分别检测VTE组（20例）和对照组（20例）受试者外周血单个核细胞（PBMC）T细胞免疫功能相关基因mRNA表达。结果VTE组患者PBMCCD247mRNA（15．3050±0．6346）、CD3DmRNA（13．7878±0．7731）、CD3GmRNA（13．3299±0．9104）、GzmAmRNA（14．8893±0．8675）、GzmBmRNA（15．9113±0．8123）、ZAP-70mRNA（14．3652±0．7717）表达水平较对照组（15．8053±0．5567、14．3820±0．4857、14．1246±0．6011、15．5305±0．4624、16．4553±0．5055、14．9229±0．4133）下调，差异均有统计学意义（P值均〈0．05）。结论VTE患者存在T细胞免疫功能紊乱，T细胞的识别抗原、传导信号功能及杀伤性病毒微生物功能下降。T细胞免疫功能紊乱有可能在VTE的发生发展中起重要作用。

Objective To explore the role of T cell-mediated immunity in the pathogenesis of venous thromboembolism （VTE） by analyzing the differential expression of T eel1 immune-related gene mRNAs peripheral blood mononuelear cells （PBMCs） between VTE patients and controls with GeneChip Human Genome. Methods Human cDNA microarray analysis was employed in PBMCs from 20 VTE patients and 20 hypertensive controls, and random variant model （RVM） corrected t-test was used for statistical analysis of differential gene expression. Results Six mRNA stripes including CD247, CD3D, CD3G, Granzyme A （GzmA） , Granzyme B （GzmB） and Zeta-chain-associated protein kinase 70 （ ZAP70 ） were found to be associated with T cell-mediated immunity. Significant down-regulation of these six mRNAs was found in the VTE group compared with the controls （15. 3050 ± 0. 6346 vs 15. 8053 ± 0. 5567, 13. 7878 ±0. 7731 vs 14. 3820 ± 0. 4857,13. 3299 ± 0. 9104 vs 14. 1246 ± 0. 6011,14. 8893 ± 0. 8675 vs 15. 5305 ±0. 4624,15. 9113 ±0. 8123 vs 16. 4553 ±0. 5055,14. 3652 ±0. 7717 vs 14. 3652 ±0. 7717;all P values 〈 0. 05 ）. Conclusions T cells＇ function including antigen recognition, signal transduction and cytotoxicity was impaired in VTE patients. T cell-mediated immunity dysfunction probably plays an important role in the pathogenesis of VTE.