量子点-RGD探针对人胰腺癌细胞株SW1990靶向标记的研究

Study on Target Marking of Quantum Dots-RGD Probe in Human Pancreatic Carcinoma Cell Line SW1990

背景:胰腺癌是一种发病隐匿、进展迅猛且预后极差的恶性肿瘤,5年生存率极低。目前靶向治疗已成为肿瘤研究的热点,量子点-RGD作为荧光探针已用于胰腺癌的靶向标记研究。目的:研究量子点-RGD荧光探针对人胰腺癌细胞株SW1990的靶向标记情况以及生物相容性。方法:常规培养胰腺癌SW1990细胞,量子点-RGD探针行靶向标记。以荧光显微镜和激光共聚焦显微镜观察10 nmol/L量子点-RGD探针对胰腺癌SW1990细胞的标记情况,MTT法检测不同浓度(0、10、15、20、40、70 nmol/L)量子点-RGD探针分别培养12 h、24 h和48 h后对胰腺癌SW1990细胞活力的影响。结果:在紫外光激发下,荧光显微镜和激光共聚焦显微镜示10 nmol/L量子点-RGD探针能有效对胰腺癌SW1990细胞进行靶向标记。与空白对照组相比,量子点-RGD探针浓度低于20 nmol/L时对胰腺癌SW1990细胞活力无影响。结论:量子点-RGD探针能有效对胰腺癌SW1990细胞进行靶向标记,日具有优良的光学特性和生物相容性,有助于胰腺癌治疗的靶向研究。

Pancreatic carcinoma is obscure in onset and progresses rapidly with poor prognosis and low five-year survival rate. Currently, targeted therapy of cancer has become a hot spot of research. Quantum dots-RGD has been used as a fluorescent probe targeting marker of pancreatic carcinoma. Aims： To study the target marking and biocompatibility of quantum dots-RGD probe in human pancreatic carcinoma cell line SW1990. Methods： Pancreatic carcinoma SW1990 cells were conventionally cultured and marked by quantum dots-RGD probe. Fluorescence microscopy and laser co^ffocal microscopy were used to observe the marking state of 10 nmol/L quantum dots-RGD probe in pancreatic carcinoma SW1990 cells. Activities of pancreatic carcinoma SW1990 cells were assessed by MTT assay after culturing with various concentrations of quantum dots-RGD probe （0, 10, 15, 20, 40, 70 nmol/L） for 12, 24, 48 hours, respectively. Results： Fluorescence microscopy and laser confocal microscopy showed that 10 nmol/L quantum dots-RGD probe could effectively target mark pancreatic carcinoma SW1990 cells. Activities of SW1990 ceils in 10, 15, 20 nmol/L quantum dots- RGD probe groups were not statistically different from that of blank control group. Conclusions：The quantum dots-RGD probe can target mark pancreatic carcinoma SW1990 cells with excellent optical specificity and biocompatibility, and would be helpful for studies on targeted therapy of pancreatic carcinoma.