水通道蛋白1、5在星形细胞瘤中的表达变化

Expression changes of aquaporin 1 and aquaporin 5 in astrocytic tumors

目的：观察水通道蛋白1、5（AQPl、5）在人不同病理级别星形细胞瘤组织中的表达差异，探讨星形细胞瘤增殖、生长的分子机制。方法：收集人各个病理级别星形细胞瘤标本55例，以肿瘤周围相对正常脑组织作为对照，采用H—E染色诊断分级，石蜡切片免疫组织化学、免疫印迹分析及逆转录聚合酶链式反应观察AQPl、5及其mRNA的表达变化。结果：与正常脑组织相比，人星形胶质瘤组织中AQPl及其mRNA表达上调，随着星形细胞瘤病理级别的升高，AQPl及其mRNA表达增强，胶质母细胞瘤组织表达最强烈；而AQP5及其mRNA仅在高恶性星形细胞瘤组织中表达增强。结论：AQPl在人星形细胞瘤组织中表达与其病理级别相关，而AQP5仅在人高恶性星形细胞瘤组织中表达增强，提示不同病理级别胶质瘤组织中AQP的表达规律不尽相同。

Objective： To investigate the expression patterns of aquaporin （AQP） 1 and AQP5 in human astrocytic tumors with different pathologic grades, and to explore the molecular mechanism underlying the growth and proliferation of astrocytoma. Methods： Expressions of AQPl and AQP5 in both mRNA and protein level in tumor tissues from 55 patients and normal brain tissues around tumors were detected by means of H-E staining, immunohistochemistry, Western blotting, and reserve transcription polymerase chain reaction. Results： Compared with normal brain tissues, the AQP1 was up-regulated in human astrocytoma, and enhanced with the increasing of pathologic grades. The strongest expression of AQP1 was found in glioblastoma. However, the up-regulated expressions of AQP5 both in mRNA and protein were only found in high-grade astrocytoma. Conclusion： The expression of AQP1 is related to the various pathologic grades of human astrocytoma, while the expression of AQP5 is up-regulated only in high-grade astrocytoma, suggesting that the expression of AQPs is different in tumor tissues of various pathologic grades.