层黏连蛋白和Ⅳ型胶原在小鼠肾发育中的表达

Expression of laminin and collagen IV in developing mouse kidney

目的：观察层黏连蛋白（LN）和Ⅳ型胶原（CoIV）在小鼠肾发育中的时空性表达，探讨其与。肾发育的关系。方法：采用免疫组织化学结合免疫印迹，检测不同胚龄及生后日龄小鼠肾组织LN和CoIV的表达及其含量变化。结果：LN在输尿管芽、各期肾小体、肾小管及集合管的基底膜处均有表达，并随肾的发育其表达量逐渐增加；CoIV在胚龄12d时，在输尿管芽周围没有表达，以后，在各期肾小体、肾小管和集合管的基底膜处均有表达，随肾的发育其表达量逐渐增加。结论：推测LN和CoIV可能对小鼠肾发育以及成熟肾各结构的维持起重要作用。

Objective： To observe the temporospatial and spatial expressions of laminin （LN） and collagen IV （Co IV ） in the developing mouse kidney and discuss its relationship with kidney development. Methods： Immunohistochemistry and Western blotting were used to detect the expression 05 LN and Co IV in embryos and postnatal mouse kidneys. Results： The expression of laminin was noticed in the ureteric bud, all stages of the glomeruli, renal tubules and collecting ducts, and increased gradually with aging; The expression of Co IV was detected in all stages of the glomeruli, renal tubules and collecting ducts, except for the ureteric bud, and also increased gradually with aging. Conclusion： LN and Co IV play an important role in the development and maturation of the mouse kidney.