小鼠肾发育中的细胞凋亡

Apoptosis during the development of the mouse kidney

目的：探讨小鼠肾发育过程中细胞凋亡的发生规律。方法：在树脂切片上，应用原位末端标记技术，观察昆明小鼠发育不同时期的肾中凋亡细胞的分布及量的变化。结果：在肾发生发育的过程中，肾皮质和髓质内几乎所有结构都有凋亡细胞散在分布。其中，肾小体的凋亡指数在生后14d达到高峰，而肾小管的凋亡指数从生后7d之后变化不大。同时，髓质中的间质细胞也发生了明显的凋亡变化。结论：伴随着肾各种结构的发生发育而出现的细胞凋亡是肾发育的一个基本特征，此过程延续到生后2周。肾单位的数量虽然在生后7d已经不再增加，但是肾小体结构的成熟和分化还在继续进行，可能将进一步促进肾小体滤过功能的成熟。

Objective： To explore the apoptosis regularity during the development of the mouse kidney. Methods： Both termi nal end-labeling technique and stereological methods were used to detect the cell apoptosis in the Kunming mice kidney tissues during the renal development and different process. Results： During the renal development, the TUNEL positive cells were found in the renal cortex and medulla, The peak intensity of apoptosis index （AI） was at postnatal day 14 （PlZt） in re nal corpuscle, while there was no peak value in the renal tubule system. The apoptosis also occurred in the interstitial cells during kidney development. Conclusion： Apoptosis is a basis feature of kidney development and this process can last postnatal 2 weeks. The number of nephron does not increase after day P7, while the structural maturation and differentiation of renal corpuscle still process, which may promote the maturation of real corpuscle filtration.