PPAR-γ和p27^(kip1)、Skp2在肝细胞癌中的表达及临床意义

The Expression and Clinical Significance of PPARγ,p27^(kip1) and Skp2 in Hepatocellular Carcinoma

目的:探讨过氧化物酶增殖物激活受体(PPAR)-γ、p27kip1和细胞S期激酶相关蛋白(Skp)2蛋白在肝癌发生发展过程中的作用。方法:肝细胞癌组织标本57例(肝癌组)和正常肝组织标本(对照组)20例。采用免疫组织化学方法检测2组PPAR-γ、p27kip1及Skp2蛋白的表达,分析它们之间及其与肝癌临床病理特征的关系。结果:肝癌组PPAR-γ及Skp2蛋白的阳性表达率高于对照组,p27kip1蛋白阳性表达率低于对照组(P<0.05),PPAR-γ蛋白与Skp2、P27kip1蛋白在不同TNM分期中的表达差异均有统计学意义(P<0.05)。PPAR-γ蛋白与Skp2、p27kip1蛋白表达无相关性(P>0.05);Skp2与p27kip1蛋白的表达呈负相关(r=-0.307,P=0.020)。Skp2蛋白低表达组的1、3、5年生存率高于高表达组(P<0.001)。结论:PPAR-γ和Skp2蛋白表达与肝细胞癌的恶性生物学行为密切相关,Skp2可作为判断肝细胞癌预后的指标。

Objective： To investigate the expression and significance of PPAR-У p27 kip1 and S-phase kinase-associated protein 2 （Skp2） in hepatocellular carcinoma. Methods： There were 57 samples of hepatocellular carcinoma （HCC group） and 20 samples of normal hepatic tissues （control group） in this study. Immunohistochemistry method was performed to detect expressions of PPAR-У, p27 kip1 and Skp2 in HCC group and control group. The relationship between expressions of PPAR-У p27 kip1 and Skp2 and clinicopathologic findings were analyzed in two groups. Results：The positive rates of PPARУ and Skp2 expression were significantly higher in HCC group than those in control group （P 〈 0.05）. The positive rate of p27 kip1 expression was significantly lower in HCC group than that in control group （P 〈 0.05）. There was significant differences for expressions of PPARУ Skp2 and p27 kip1 in different TNM stages （P 〈 0.05）. There was no significant correlation between PPAR-У Skp2 and p27 kip1 （P 〉 0.05）. The positive correlation was found between Skp2 and P27 kip1 expression （r = -0.307, P = 0.020）. The one, two and five-year survival rates were significantly higher in patients with low-expression level of Skp2 than those of patients with high-expression level of Skp2 （P 〈 0.001）. Conclusion： The expressions of PPAR-У and Skp2 are associated with aggressive tumor behavior of HCC. The expression of Skp2 might serve as the key prognostic factors in HCC.