酿酒酵母Sfi1p的结构及生物学功能

The Structure and Biological Functions of Sfi1p in Yeast Saccharomyces cerevisiae

纺锤体极体(spindle pole body,SPB)是酵母细胞的微管组织中心,它在细胞分裂及细胞遗传稳定性的维持过程中起着极其重要的作用,是细胞生物学领域热门的研究方向.Sfi1p是酿酒酵母SPB的必需蛋白并且横跨整个半桥,该蛋白与SPB的复制有关,它的缺失或突变会导致SPB复制失败,在哺乳动物的中心体也存在酵母Sfi1p的同源蛋白.本文系统的介绍了酵母Sfi1p及其在人类中心体中的同源蛋白hSfi1p的结构特征,并且阐明了Sfi1p在SPB复制与分离、核配及生孢等细胞周期过程中的作用.对Sfi1p的功能研究,将有助于解决SPB研究过程中重要的科学问题,同时为中心体中Sfi1p同源蛋白的功能研究提供良好的借鉴.

As the microtubule organizing center of yeast cells,the spindle pole body（SPB） plays an important role in cell division and genome stability,therefore attracting a great attention and enormous research efforts on its physiological roles.In the yeast Saccharomyces cerevisiae,Sfi1p is an essential component of SPB,which localizes at half-bridge and spans its full length.Sfi1p,whose deletion or mutation causes a failure of SPB replication,is required for SPB duplication and self-assembly.A large number of studies show that Sfi1p has its homologous proteins in the mammalian centrosome.In this study,we reviewed the general structure of Sfi1p for both SPB in Saccharomyces cerevisiae and centrosome in human and elucidated the global function of Sfi1p involved in the cellular processes,including SPB duplication,separation,karyogamy and sporulation process.Studies on Sfi1p function will not only shed new light on our current understanding about yeast SPB structure and function,but also lay a foundation for investigation of its homologues in the mammalian centrosome.