TGF—β／Taq基因多态性与广东人瘢痕疙瘩相关性的研究

Relationship between gene polymorphism of TGF-β/Taq and keloid of cantonese

目的探讨广东人瘢痕疙瘩与转化生长因子-β（transforming growthfactor—β，TGF—β）基因单核苷酸多态性的关系。方法应用聚合酶链反应．限制性片段长度多态性核苷酸分型技术，以Traq限制性内切酶消化PCR扩增产物，对43例广东人瘢痕疙瘩及43例正常皮肤的TGF-β／Taq等位基因进行分析。结果瘢痕疙瘩的C／C等位基因频率较正常皮肤对照组明显增高，差异有统计学意义（P〈0．05）；有家族史瘢痕疙瘩患者的TGF-β／Taq基因型与无家族史患者比较，差异有统计学意义（P〈0．05）。结论TGF-β／Taq基因多态性与广东人瘢痕疙瘩的发生有关；有瘢痕疙瘩家族史可能与TGF-β／Taq少见变异体有关。

Objective To study the relationship between single nucleotide polymorphism of TGF-β/Taq and keloid of Cantonese. Methods TGF-β/Taq allelomorph in 43 patients with keloid as the experimental group and 43 people with normal skin as the control group were analyzed by PCR-RELP through PCR amplification digested by Traq restriction enzyme. Results The C/C allele frequency of TGF-β in the experimental group was significantly higher than that in the control group, the difference was significant （ P 〈 0.05 ） ; compared with the people in the control group, the TGF-β genotype in the experimental group was significantly higher, the difference was significant （ P 〈 0.05 ）. Conclusion The development of keloid of Cantonese is related with gene polymorphism of TGF-β ; A family history of keloid may correlate with the TGF-β variant.