增生性瘢痕形成过程中组织谷胱甘肽和辅酶Ⅱ含量变化研究

Variation of glutathione and coenzyme Ⅱ in tissues during formation of hyperplastic scar

目的探讨瘢痕增生过程中氧化还原状态的动态变化情况。方法对正常皮肤、伤后2周组织（早期组）、伤后1个月组织（中期组）和伤后6个月组织（成熟期组）中的氧化型与还原型谷胱甘肽（GSSG、GSH）、氧化型与还原型辅酶Ⅱ（NADP^＋、NADPH）含量进行测定，计算还原型与氧化型的比值，分析氧化还原状态的变化。结果各个时期烧伤组织中GSSG、GSH、NADP^＋、NADPH的含量均高于正常皮肤组（P〈0．05）；不同阶段烧伤组织中GSSG、GSH、NADP^＋、NADPH的含量随时间延长而下降，各组之间各项指标差异有统计学意义（P〈0．05）；早期组GSH／GSSG明显低于其余各组，其余各组间GSH／GSSG、NADPH／NADP^＋的值，其差异无统计学意义（P〉0．05）。结论组织微环境内高氧化还原状态可能是病理性瘢痕发生机制中的一个重要环节，随着瘢痕的成熟其组织内的氧化还原水平下调，但仍高于正常皮肤组织的氧化还原水平。

Objective To investigate the changes of the redox state of scars during scar hyperplasia. Methods Contents of oxidized and reduced glutathione, coenzyme Ⅱ （ GSSG, GSH, NADP ^＋ , NADPH） were detected in the tissues of normal skin, scar of the early stage （2 weeks after burn）, scar of mid-stage （ lmonth after burn）, and scar of stable stage（ ≥6 months after burn）, and the ratio of oxidized and reduced forms was calculated and the changes of redox state was analyzed. Results Compared with normal skin tissue, the contents of GSSG, GSH, NADP^＋ , NADPH were significantly higher in scars（P〈0.05） ; as time increases, the contents of GSSG, GSH, NADP^ ＋ , NADPH decreased, and the difference among the scar at different stages were significant （ P 〈 0.05 ） ; the contents of GSH/GSSG were more lower than those in the other groups, there was no significant difference observed on the ratio of GSH/GSSG, NADPH/NADP ^＋ in the other groups（ P 〉 0.05 ）. Conclusion The high redox state in microenvironment may play a key role in the pathogenesis of pathological scar, and the redox state may step down along with the process of scar maturation but the level of redox state is still higher than that in the normal skin.