摘要
A method for separation of glucose oxidase(GOD) has been developed. A chroma tographic column is filled with an anion exchanger of styrene divinyl benzene matrix conta ining quaternary ammonium salt. The buffered phosphate and acetate solutions were used as eluents. Two GOD components(GOD A and GOD B) were separated by this method. Their enzyme activity and ζ potential values were 86 9, 27 3, and -4 3 mV, -6 4 mV, respecti vely . The simplicity and low cost of the method make it valuable for practical applications.
A method for separation of glucose oxidase(GOD) has been developed. A chroma tographic column is filled with an anion exchanger of styrene divinyl benzene matrix conta ining quaternary ammonium salt. The buffered phosphate and acetate solutions were used as eluents. Two GOD components(GOD A and GOD B) were separated by this method. Their enzyme activity and ζ potential values were 86 9, 27 3, and -4 3 mV, -6 4 mV, respecti vely . The simplicity and low cost of the method make it valuable for practical applications.
出处
《应用化学》
CAS
CSCD
北大核心
2000年第2期171-173,共3页
Chinese Journal of Applied Chemistry
基金
国家自然科学基金资助课题! ( 2 95 73 13 9)
关键词
葡萄糖果氧化酶
分离
活性
构象
glucose oxidase,separation,enzymatic activity
