摘要
目的建立高效液相色谱法测定祛风活络膏贴制剂中葛根素含量的方法。方法采用ZORBAX SB-C18色谱柱(4.6mm×250mm,5μm);流动相为甲醇∶水(26∶74);流速为1 mL/min;检测波长为250 nm;柱温为25℃。结果葛根素含量在0.0604μg-0.604μg(r=0.9998)范围里呈良好线性关系。平均加样回收率为98.75%(RSD=0.72%)。3批样品中,葛根素含量分别为4.53、4.53、4.54 mg/g。结论该方法简便、准确,可靠,可用于祛风活络膏贴中葛根素含量测定。
Objective To establish a HPLC method to determine the content of puerarin in Qufeng Huoluo patch. Methods The col- umn of Zorbax SB - C18 (4.6mm × 250mm, 5 μm)was used. The mobile phase was composed of methanol -water (26: 74) and the flow rate was 1 mL/min. The detection wavelength was at 250 nm and the column temperature was 25℃. Results The linear range of puerarin was 0. 0604 - 0. 604μg( r = 0. 9998 ) and the linear relationship was good. The average recovery was 98.75% and the RSD was 0. 72%. In three batches of sample, the contents of puerarin were 4.53,4.53,4.54 mg/g respectively. Conclusion The method is simple, accurate, and reli- able and can be used for the determination of the content of in Qufeng Huoluo patch.
出处
《湖北中医药大学学报》
2012年第3期23-24,共2页
Journal of Hubei University of Chinese Medicine