转GmHSFA1基因大豆的耐热性评价

Heat Resistance Evaluation of GmHSFA1 Transgenic Soybean

在高温胁迫条件下对11个转GmHSFA1基因的大豆品系及其受体的GmHSFA1及靶基因GmHSP70、GmHSP22和GmHSP17.9的表达和生理生化、光合特性及产量性状变化进行了研究,并采用胁迫系数与灰色关联分析相结合方法,对转基因大豆材料耐高温能力进行综合评价。结果表明:多数转基因大豆品系在常温和热诱导条件下GmHSFA1及靶基因GmHSP70、GmHSP22和GmHSP17.9表达水平明显高于非转基因受体;高温处理后,绝大多数转基因材料的脯氨酸含量增幅都要明显高于非转基因受体,丙二醛含量除HTH-4以外增幅均小于非转基因受体,可溶性糖含量增幅全部高于非转基因受体材料。多数转基因材料的光合性状与产量性状降幅均小于非转基因受体。关联度分析表明与非转基因受体相比,有9个转GmHSFA1基因品系的耐热能力得到明显提高。

In this study,the expression of GmHSFA1,GmHSP70,GmHSP22 and GmHSP17.9 in 11 GmHSFA1 transgenic soybean lines and their receptor were evaluated and researched the change in physiological indices（Pro,MDA,Wss）,photosynthetic characteristics（Pn,Cond,Ci,Tr）and yield after high temperature treatment.In most of the transgenic soybean lines,the expression of GmHSFA1、GmHSP70、GmHSP22 and GmHSP17.9 were higher than non-transgenic receptor at 28℃ and 48℃.After heat treatment,the increment of proline content and soluble sugar in most transgenic soybean lines were clearly higher than non-transgenic receptor,increment of MDA content were lower than receptor except HTH-4,the decrement of photosynthetic traits and yield in most of the transgenetic materials were less than non-transgenic receptor.Through gray relation analysis,we concluded that the heat resistance of 9 GmHSFA1 transgenic lines were improved significantly than non-transgenic receptor.