摘要
以大豆品种"铁丰31"为试材,在幼苗第二片复叶完全展开时分别以1 500 mg.L-1的KHCO3和NaHCO3喷施于叶片的正反两面,测定光合速率、可溶性糖、光合色素等生理指标,以探讨K+、Na+和HCO3-对光合作用的影响。结果表明:与清水对照处理相比,K+、Na+和HCO3-能提高大豆幼苗的光合速率、可溶性糖含量、叶绿素a、b和叶绿素总含量,增加叶绿素b在总叶绿素中的比例;促进光反应的电子传递为暗反应碳固定提供更多的同化力来促进大豆幼苗光合作用;提高大豆幼苗叶片的ATP合酶活性及光合磷酸化活性;增加大豆幼苗叶片PEPC酶活力和PEPC比活力;其中HCO3-对提高幼苗光合速率、叶绿素b合成及全链光合电子传递速率起到显著促进作用;NaHCO3显著增加了Rubisco含量。K+对Rubisco含量的促进作用小于Na+,对其它各指标的促进作用均大于Na+。
In this study,seedling of Tiefeng 31 were sprayed with 1500 mg·L-1 KHCO3 and NaHCO3,respectively,and determined the photosynthetic rate,soluble sugar,photosynthetic pigment and other physiological indexes to explore the effect of K＋,Na＋ and HCO-3on photosynthesis of soybean seedlings.The results showed that：compared with water spraying control,K＋,Na＋ and HCO-3improved photosynthetic rate,the content of soluble sugar,chlorophyll a,chlorophyll b,and the ratio of chlorophyll b in total chlorophyll;promoted soybean seedlings photosynthesis by providing more assimilatory power to dark reactions carbon fixation;and enhanced the activities of ATP enzyme,photophosphorylation and PEPC.In addition,HCO-3played a more important role in promoting chlorophyll b synthesis and improving photosynthetic rate.NaHCO3 significantly increased the content of Rubisco.For other physiological indexes but the content of Rubisco,the promotion effect of K＋ was higher than Na＋.
出处
《大豆科学》
CAS
CSCD
北大核心
2012年第3期436-439,共4页
Soybean Science
基金
辽宁省教育厅科学技术研究项目(2004A011)
