富硒豆的培养及其硒含量分析

Cultivation of Selenium-enriched Legumina and Analysis of Its Selenium Content

为了开发新型富硒功能食品———富硒豆,研究了绿豆和大豆在不同含硒溶液中发芽生长情况。结果表明,20.00#g.mL-1的高浓度硒培养溶液会对绿豆和大豆的发芽生长产生明显毒性,且四价硒毒性更强,表现在四价硒培养的绿豆和大豆发芽率较六价硒低;分别在0～3.00#g.mL-1和0～6.00#g.mL-1的六价硒溶液培养下,绿豆和大豆发芽生长情况基本良好。采用氢化物原子荧光光谱法作外标标准曲线,并测定培养后的绿豆和大豆硒含量,结果20#g.mL-1的高浓度硒溶液培养的豆含硒量最高,为75.0273～102.2612#g.L-1。而在0～6.00#g.mL-1的六价硒培养条件下,绿豆和大豆的含硒量分别为7.5002～69.8483#g.L-1和11.1737～58.3926#g.L-1,且该硒含量范围适合富硒功能食品硒含量标准。

The mungbean and soybean germinating in different Selenium cultivating solution were investigated for developing Selenium-enriched legumina as rich Selenium foodstuff.The mungbeans and soybeans can be toxic significantly during germination in Selenium solution of high concentrations as 20.00μg·mL-1.The germination rate of mungbean and soybean cultivating in tetravalent Selenium（SeⅣ） was lower than that in hexavalent Selenium（SeⅥ）.Therefore,the SeⅣ was more toxic than that of SeⅥ.When the SeⅥ concentration below 3.00 μg·mL-1,the mungbeans buds could grow better,while the SeⅥ concentration below 6.00 μg·mL-1,the soybeans could grow better.Hydride generation-atomic fluorescence spectrometry（HG-AFS）was applied to make the external regression curve,and the Selenium content of mungbean and soybean was determined by this approach.The results indicated that when the legumina was,cultivated in high Selenium concentration as 20μg·mL-1,the detected Selenium was in the range of 75.0273-102.2612 μg·L-1.When cultivated in SeⅥ concentrations below 6.00 μg·mL-1,the Selenium content for mungbean and soybean was about 7.5002-69.8483 μg·L-1 and 11.1737-58.3926 μg·L-1,respectively,which could meet the national standard of Selenium-enriched foodstuff.