铁炮百合的胚性愈伤组织诱导和植株再生

EMBRYONIC CALLUS INDUCTION AND PLANT REGENERATION OF Lilium longiflorum

以铁炮百合(又称麝香百合)杂种系品种‘White heaven'的组培苗叶片为外植体,研究了不同种类和浓度的激素对愈伤组织诱导和植株再生的影响,并通过叶片位置、接种方式以及光暗等不同处理进一步优化胚性愈伤组织的诱导。结果表明:以组培苗基部叶片为外植体,采用叶片近轴面向上的接种方式,在MS+1.0mg.L-12,4-D+0.1mg.L-16-BA的培养基上暗培养可诱导出胚性愈伤组织,诱导率可达65.74%;经石蜡切片鉴定可观测到球形胚和心形胚。最佳分化和生根培养基分别为MS+0.1mg.L-1NAA和1/2MS+0.5mg.L-1IBA,分化率达81.48%,生根率达91.67%。结果还显示2,4-D在百合胚性愈伤组织的诱导中起到了关键作用。

The effects of different kinds and concentrations of plant growth regulator on callus induction and plantlet regeneration were investigated with leaves of seedling of Lilium longiforum ’white heaven’ from tissue culture as explants.The induction of embryonic callus was further optimized by treatments of different parts of leaves,inoculating ways and light conditions.The results showed that using the base of leaves and inoculating on medium with abaxial side during cultivation,the rate of callus induction was 65.74% in dark treatment on the medium modified MS with 1.0mg/L 2,4-D and 0.1mg/L 6-BA.Globular embryo and heart stage embryo were observed by paraffin section identification.The best mediums for differentiation and rooting were modified MS medium with 0.1mg/L NAA and 1/2MS with 0.5mg/L IBA respectively,differentiation rate was 81.48%,and rooting rate was 91.67%.The results also indicated that 2,4-D played a key role in embryonic callus induction of Lily.