姜辣素对^(60)Co γ射线照射小鼠造血和抗氧化损伤的治疗作用研究

THERAPEUTIC EFFECTS OF GINGEROL ON HEMATOPOIETIC AND ANTIOXIDATIVE DAMAGE OF ^(60)Co γ-RAYS IRRADIATED MICE

研究了姜辣素对60Coγ射线损伤小鼠造血和抗氧化功能的治疗作用。18只雌性健康昆明小鼠被随机分为3组,每组6只,分别为对照组、照射组、照射给药组。对照组不照射,连续灌胃5d蒸馏水;照射组和照射给药组分别用3Gy60Coγ射线进行照射,并分别在照射后30min内灌胃蒸馏水和姜辣素,连续5d。末次灌胃后48h内测定所有小鼠血液中白细胞(WBC)和红细胞(RBC)数量、脏器指数、肝脏超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)、丙二醛(MDA)含量及骨髓嗜多染红细胞微核(MN)数目。与对照组相比,照射组脾脏指数、WBC极显著降低(P<0.01),肝脏MDA含量、骨髓MN数目极显著升高(P<0.01);与照射组相比,照射给药组脾脏指数、T-AOC、SOD活性极显著升高(P<0.01),肝脏指数、WBC、RBC含量也有所升高,MDA含量、MN数目极显著降低(P<0.01)。结果表明,姜辣素对60Coγ射线照射造成的小鼠造血、抗氧化功能损伤具有治疗作用。

18 female Kunming mice were chosen and randomly divided into three groups,and the therapeutic effects of gingerol on hemopoietic and antioxidative system in liver of 60Co γ-rays irradiated mice were developed in this study.Control group was given distilled water intragastrically once a day for five days.Mice in the irradiated group and irradiated＋gingerol group were both irradiated at 3Gy of 60Co γ-rays and were given distilled water and gingerol intragastrically within 30min after irradiation respectively,once a day for five days.The mice were sacrificed and sampled in 48 hours after intragastric administration.Compared with control group,the relative spleen index and WBC numbers significantly decreased（P〈0.01）,and MDA content and MN numbers in bone marrow increased（P〈0.01）in irradiated group.The irradiated ＋ gingerol group showed significantly higher spleen index,T-AOC and SOD activities（P〈0.01）,lower MDA contents and MN numbers（P〈0.01）compared with irradiated group.WBC and RBC numbers in irradiated ＋ gingerol group were also higher than those in irradiated group.The results indicated that the gingerol has the therapeutic effects on hematopoietic and antioxidative damage of 60Co γ-rays irradiated mice.